The pre-implantation embryo in a competent status and post-implantation fully differentiation of the inner cell mass (ICM) and trophectoderm (TE) are prerequisites of successful implantation. Type I embryonic epithelial-mesenchymal transition (EMT) involves in these processes. A high level of the mir-let-7 family was found in the dormant mouse embryo of implantation failure in our previous study. Besides, its natural inhibitor lin28a was found to function in maintained stem cell pluripotency and involved in early embryo nucleolus construction. Until now, few studies got involved in the exact molecular mechanism that affects embryo implantation potential. In this study, the possible function of Wnt/β-catenin-lin28a/let-7 pathway in mouse embryo implantation was studied. ICR mouse, Lin28a/Let-7 g transgenic mice (Lin28a-TG/Let-7 g-TG), and implanting dormant mice models were used for the study. Wnt/β-catenin signaling is essential in embryo implantation, which promotes embryo implantation through directly trigger lin28a expression, thus represses the mir-let-7 family. Lin28a and mir-let-7 both participate in implantation via an inverse function. Lin28a and mir-let-7 participate in embryo implantation through embryonic EMT. Wnt/β-catenin signaling promotes embryo implantation and accompanying embryonic EMT, which is mediated by directly activate lin28a/let-7 axis.

中文翻译：

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Wnt/β-catenin-lin28a/let-7 轴在胚胎着床能力和上皮间质转化 (EMT) 中的作用。

处于有能力状态的植入前胚胎和植入后内细胞团 (ICM) 和滋养外胚层 (TE) 的完全分化是成功植入的先决条件。I 型胚胎上皮间质转化 (EMT) 参与这些过程。在我们之前的研究中，在植入失败的休眠小鼠胚胎中发现了高水平的 mir-let-7 家族。此外，发现其天然抑制剂 lin28a 可维持干细胞多能性并参与早期胚胎核仁构建。到目前为止，很少有研究涉及影响胚胎植入潜力的确切分子机制。本研究研究了Wnt/β-catenin-lin28a/let-7通路在小鼠胚胎植入中的可能作用。ICR 小鼠、Lin28a/Let-7 g 转基因小鼠 (Lin28a-TG/Let-7 g-TG)、并植入休眠小鼠模型用于研究。Wnt/β-catenin 信号在胚胎着床中是必不可少的，它通过直接触发 lin28a 表达促进胚胎着床，从而抑制 mir-let-7 家族。Lin28a 和 mir-let-7 都通过反函数参与植入。Lin28a 和 mir-let-7 通过胚胎 EMT 参与胚胎植入。Wnt/β-catenin 信号促进胚胎植入和伴随胚胎 EMT，这是通过直接激活 lin28a/let-7 轴介导的。Lin28a 和 mir-let-7 通过胚胎 EMT 参与胚胎植入。Wnt/β-catenin 信号促进胚胎植入和伴随胚胎 EMT，这是通过直接激活 lin28a/let-7 轴介导的。Lin28a 和 mir-let-7 通过胚胎 EMT 参与胚胎植入。Wnt/β-catenin 信号促进胚胎植入和伴随胚胎 EMT，这是通过直接激活 lin28a/let-7 轴介导的。