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CaHsp26.5 promotes defense responses against RNA viruses via ATAF2 but is hijacked as a chaperone for tobamovirus movement protein.
Journal of Experimental Botany ( IF 6.9 ) Pub Date : 2020-07-08 , DOI: 10.1093/jxb/eraa320
Siew-Liang Foong 1 , Kyung-Hee Paek
Affiliation  

Abstract
The expression of Capsicum annuum HEAT SHOCK PROTEIN 26.5 (CaHsp26.5) was triggered by the inoculation of Tobacco mosaic virus pathotype P0 (TMV-P0) but its function in the defense response of plants is unknown. We used gene silencing and overexpression approaches to investigate the effect of CaHsp26.5 expression on different plant RNA viruses. Moreover, we performed protein–protein and protein–RNA interaction assays to study the mechanism of CaHsp26.5 function. CaHsp26.5 binding to a short poly-cytosine motif in the 3'-untranslated region of the genome of some viruses triggers the expression of several defense-related genes such as PATHOGENESIS-RELATED GENE 1 with the help of a transcription factor, NAC DOMAIN-CONTAINING PROTEIN 81 (ATAF2). Thus, an elevated CaHsp26.5 level was accompanied by increased plant resistance against plant viruses such as Cucumber mosaic virus strain Korea. However, the movement proteins of Pepper mild mottle virus pathotype P1,2,3 and TMV-P0 were shown to be able to interact with CaHsp26.5 to maintain the integrity of their proteins. Our work shows CaHsp26.5 as a positive player in the plant defense response against several plant RNA viruses. However, some tobamoviruses can hijack CaHsp26.5’s chaperone activity for their own benefit.


中文翻译:

CaHsp26.5通过ATAF2促进针对RNA病毒的防御反应,但被劫持为烟草花叶病毒运动蛋白的伴侣。

摘要
辣椒花叶病毒病原菌P 0(TMV-P 0)的接种触发了辣椒辣椒热休克蛋白26.5(CaHsp26.5)的表达,但其在植物防御反应中的功能尚不清楚。我们使用基因沉默和过度表达的方法来研究CaHsp26.5表达对不同植物RNA病毒的影响。此外,我们进行了蛋白质-蛋白质和蛋白质-RNA相互作用测定以研究CaHsp26.5功能的机制。CaHsp26.5与某些病毒基因组3'-非翻译区中的短多胞嘧啶基序结合,触发了几种与防御相关的基因的表达,例如与病原相关的基因1借助转录因子NAC DOMAIN-CONTAINING Protein 81(ATAF2)。因此,升高的CaHsp26.5水平伴随着对植物病毒如黄瓜花叶病毒株韩国的植物抗性增强。然而,显示出胡椒轻斑驳病毒致病型P 1,2,3和TMV-P 0的运动蛋白能够与CaHsp26.5相互作用,以维持其蛋白的完整性。我们的工作表明,CaHsp26.5在针对几种植物RNA病毒的植物防御反应中发挥积极作用。但是,某些烟草花叶病毒可以利用CaHsp26.5的伴侣活性来劫持自己的利益。
更新日期:2020-10-08
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