Biomolecules function in an aqueous environment. Elucidation of the hydration structures of biomolecules is hence important to understand their functions. Here, we investigated the hydration structure of lysozyme (Lys) in the gas phase by photodissociation and fluorescence spectroscopy in combination with droplet‐beam laser ablation mass spectrometry. We found that water molecules are held inside and on the surface of the Lys molecule, and the hydration structure around the tryptophan residue changes by photoexcitation. This study provides a novel method to observe the hydration structures of large biomolecules at the molecular level.

中文翻译：

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通过光解和荧光光谱研究液滴束的红外激光烧蚀产生的溶菌酶离子的气相水合

生物分子在水性环境中起作用。因此，阐明生物分子的水合结构对于理解它们的功能很重要。在这里，我们通过光解和荧光光谱结合液滴束激光烧蚀质谱法研究了气相中溶菌酶（Lys）的水合结构。我们发现水分子被固定在赖氨酸分子的内部和表面，并且色氨酸残基周围的水合结构因光激发而发生变化。该研究为在分子水平上观察大生物分子的水合结构提供了一种新方法。