6″-O-Succinyldaidzin is a daidzin derivative in natto with little bioactivity reports. We investigated the anti-ischemic effects and underlying mechanisms of 6″-O-Succinyldaidzin in MCAO model and used MRI to measure the infarct volume, and neurological scores to evaluate the effect of the injury after occlusion for 2 h and reperfusion for 24 h on male rats. Levels of Nrf2, Keap1, HO-1, ERK were measured by immunoblots to study the underlying mechanisms of 6″-O-Succinyldaidzin activity. Further, we confirmed our results in HT-22 cells using immunofluorescence to assay for the expression of Nrf2 and Keap1. 6″-O-Succinyldaidzin reduced both infarct areas and neurological scores of MCAO rats. Levels of ERK, HO-1, and nuclear-Nrf2 were upregulated after the administration of 6″-O-Succinyldaidzin. Immunofluorescence assays indicated that Nrf2 expressed in the nucleus while Keap1 remained in the cytoplasm after treatment with 6″-O-Succinyldaidzin. Our study shows that 6″-O-Succinyldaidzin of natto elicits an anti-ischemic effect and activates Nrf2-related antioxidant.

6″ -O-琥珀酰黄豆苷是纳豆中的黄豆苷衍生物，几乎没有生物活性报道。我们研究了6” -O-琥珀酰大豆苷在MCAO模型中的抗缺血作用及其潜在机制，并使用MRI来测量梗塞体积，并用神经学评分评估闭塞2 h和再灌注24 h后的损伤效果。雄性大鼠。通过免疫印迹法检测Nrf2，Keap1，HO-1，ERK的水平，以研究6″ -O-琥珀酰黄豆苷活性的潜在机制。此外，我们在HT-22细胞中使用免疫荧光检测Nrf2和Keap1的表达来证实我们的结果。6″ -O-琥珀酰黄豆苷减少了MCAO大鼠的梗塞面积和神经学评分。施用6″ -O-琥珀酰黄豆苷元后，ERK，HO-1和核Nrf2的水平上调。免疫荧光分析表明，Nrf2在6” -O-琥珀酰黄豆苷处理后在细胞核中表达，而Keap1保留在细胞质中。我们的研究表明，纳豆的6''-O-琥珀酰黄豆苷具有抗缺血作用并激活Nrf2相关的抗氧化剂。