Introduction

Prompt detection of antibiotic resistance genes in healthcare institutions is of utmost importance in tackling the spread of multi-drug resistant micro-organisms. We evaluated the Antimicrobial Resistance (AMR) Direct Flow Chip Kit versus phenotypic screening assays for rectal and nasopharyngeal specimens upon ICU admission.

Methods

A total of 184 dual specimens (92 rectal and 92 nasopharyngeal swabs) from 92 patients were collected from 11/2017 to 8/2018. All swabs were subjected to both AMR and phenotypic tests according to their origin. The degree of agreement of the two methods was assessed by the kappa coefficient.

Results

The kappa coefficient showed perfect agreement for MRSA, ESBLs, oxacillinases and vancomycin resistance genes (1.000, p < 0.01) and very good agreement for mecA-positive CoNS, KPC-carbapenemases and metallo-beta-lactamases (0.870, p < 0.01; 0.864, p < 0.01; and 0.912, p < 0.01, respectively).

Conclusion

The AMR Direct Flow Chip Kit is a useful alternative to phenotypic testing for rapid detection of resistance markers.

中文翻译：

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“AMR Direct Flow Chip Kit”DNA微阵列在ICU入院时直接从直肠和鼻咽临床样本中检测抗菌素耐药基因的评价

介绍

及时检测医疗机构中的抗生素耐药基因对于应对多重耐药微生物的传播至关重要。我们在入住 ICU 时评估了抗微生物药物耐药性 (AMR) 直接流式芯片试剂盒与直肠和鼻咽标本的表型筛查分析。

方法

2017 年 11 月至 2018 年 8 月，共收集了 92 名患者的 184 个双重标本（92 个直肠和 92 个鼻咽拭子）。所有拭子都根据其来源进行了 AMR 和表型测试。两种方法的一致程度通过kappa系数进行评估。

结果

Kappa 系数与 MRSA、ESBL、苯唑西林酶和万古霉素抗性基因完全一致（1.000，p  <  0.01），并且与mecA阳性 CoNS、KPC-碳青霉烯酶和金属-β-内酰胺酶非常一致（0.870，p  <  0.01；0.864） , p  <  0.01; 和 0.912, p  <  0.01)。

结论

AMR Direct Flow Chip Kit 是表型测试的有用替代品，可用于快速检测耐药标记。