The central nervous system (CNS) injury has been a worldwide clinical problem for regenerative medicine. Nerve tissue engineering is a new strategy for CNS injury. Among kinds of biomaterials, graphene oxide (GO)-based degradable composite materials are considered to be promising in the field of neurogenesis. In this study, GO and L-theanine (TH) were combined by chemical grafting to prepare a new PLGA/GO-TH composite material. X-ray diffraction (XRD), transmission electron microscope (TEM), Fourier-transform infrared spectra (FTIR), contact angle testers, and mechanical testers were performed to obtain characterization of composite materials. The protein adsorption efficiency of the PLGA/GO-TH films was then evaluated. Next, the effect of the composite films on neural stem cell (NSC) survival, proliferation, and differentiation was investigated. Our results indicated that L-theanine was successfully grafted onto GO. PLGA/GO-TH composite film can significantly improve NSC survival, proliferation, and neuronal differentiation. Our results demonstrated that the neurogenesis function of a novel PLGA/GO-TH composite film and its potential as a carrier for the further application in the CNS injury.

中文翻译：

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茶氨酸修饰的氧化石墨烯复合膜用于神经干细胞的增殖和分化

中枢神经系统（CNS）损伤已成为再生医学的全球性临床问题。神经组织工程是中枢神经系统损伤的新策略。在各种生物材料中，基于氧化石墨烯（GO）的可降解复合材料被认为在神经发生领域很有前途。在这项研究中，GO和L-茶氨酸（TH）通过化学接枝结合在一起以制备新型PLGA / GO-TH复合材料。进行了X射线衍射（XRD），透射电子显微镜（TEM），傅立叶变换红外光谱（FTIR），接触角测试仪和机械测试仪，以表征复合材料。然后评估PLGA / GO-TH膜的蛋白质吸附效率。接下来，研究了复合膜对神经干细胞（NSC）存活，增殖和分化的影响。我们的结果表明，L-茶氨酸已成功嫁接到GO上。PLGA / GO-TH复合膜可以显着改善NSC的存活，增殖和神经元分化。我们的结果表明，新型PLGA / GO-TH复合膜的神经发生功能及其作为载体进一步应用于中枢神经系统损伤的潜力。