Abstract

Present study is engrossed in identification of phyto-constituents from aerial part extracts of Tamarix gallica and appraisal of its anti-oxidant, anti-diabetic and anti-inflammatory potential based upon its folktale use. The methanol and n-hexane fractions of aerial parts were analysed using high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) respectively. Inhibitory concentration (IC₅₀) showed better results in case of methanolic extract for both in antioxidant (IC₅₀= 15.47 µg/mL) and alpha amylase (IC₅₀=18.75 µg/mL) assays. Significantly higher quantities of phenolic and flavonoid contents were present in methanolic extract. A significant correlation was found to be existed between these contents and IC₅₀ of antioxidant assay. Alloxan induced hyperglycaemia declined along with improvement in lipid profile, C-reactive proteins (CRP), liver function tests (LFTs) and renal function tests (RFTs). Methanolic fraction (500 mg/kg) was also related to significant reduction in levels of inflammatory markers i.e. tumour necrosis factor-alpha, TNF- α (1.28 ± 0.13 g/L) and interleukin-6, IL-6 (98 ± 10.4 pg/L) as observed in diabetic rats. Based upon the above findings, the study suggests that methanolic fraction from aerial parts of the T. gallica has better anti-diabetic profile which might be attributed to its alpha amylase, anti-oxidant and anti-inflammatory potential.

摘要

目前的研究专注于从柽柳地上部分提取物中鉴定植物成分，并根据其民间故事用途评估其抗氧化、抗糖尿病和抗炎潜力。分别使用高效液相色谱 (HPLC) 和气相色谱-质谱 (GC-MS) 分析地上部分的甲醇和正己烷馏分。在抗氧化剂 (IC ₅₀ = 15.47 µg/mL) 和 α 淀粉酶( _{IC 50}=18.75 µg/mL) 测定。甲醇提取物中酚类和类黄酮含量显着增加。发现这些含量与抗氧化测定的IC ₅₀之间存在显着相关性。随着血脂、C 反应蛋白 (CRP)、肝功能测试 (LFT) 和肾功能测试 (RFT) 的改善，四氧嘧啶诱导的高血糖症下降。甲醇部分 (500 mg/kg) 也与炎症标志物水平显着降低有关，即肿瘤坏死因子-α、TNF-α (1.28 ± 0.13 g/L) 和白细胞介素-6、IL-6 (98 ± 10.4 pg) /L) 如在糖尿病大鼠中观察到的。基于上述发现，该研究表明来自T. gallica地上部分的甲醇部分具有更好的抗糖尿病特性，这可能归因于其α淀粉酶、抗氧化和抗炎潜力。