ABSTRACT

Trichinellosis is a food-borne disease caused by the ingestion of meat infected with Trichinella spiralis encysted larvae. To interrupt the circle between human and animals, proper immunization and treatment of animals are needed. This study aimed to compare the impact of vaccination with gamma radiation-attenuated larvae with anti-helminthes activity of Punica granatum on its control. Sixty mice were divided into six groups as follows: normal control, infected control, vaccine control, vaccinated-challenged, treatment-control and infected-treated. Parasitological assessment and immunohistochemical expression of GATA-3 in muscle tissues were done. The serum level of 8-hydroxy-2-de-axyguanine, an oxidative stress marker, as well as flow cytometric analysis of splenic CD4+ and CD8+ subsets were determined. Results exhibited high significant reduction in the muscle larvae in both vaccinatedchallenged (72.5 %) and infected-treated group (56.3 %) with reduced 8-OHdG and CD4+ and CD8+ cells. Also, moderate expression of GATA-3 in infected-treated group and minimal or no expression in vaccinated-challenged was determined. It could be concluded that vaccination with radiation-attenuated larvae elicits immune protection against challenged infection. Also, treatment with Punica granatum has therapeutic activity against infection. However, better effect was achieved by irradiated vaccine so Punica granatum can be used as an adjuvant with vaccination for complete protection.

摘要

旋毛虫病是一种食源性疾病，是由于食入感染旋毛虫的幼虫感染的肉引起的。为了打断人与动物之间的圈子，需要对动物进行适当的免疫和治疗。这项研究旨在比较接种γ射线的减毒幼虫对石榴的抗蠕虫活性的影响。在其控制之下。60只小鼠分为以下六组：正常对照，感染对照，疫苗对照，接种疫苗攻击，治疗对照和感染处理。进行了肌肉组织中GATA-3的寄生虫学评估和免疫组化表达。确定了血清8-羟基-2-去轴鸟嘌呤的水平，一种氧化应激标记物，以及脾脏CD4 +和CD8 +亚群的流式细胞仪分析。结果显示，经免疫接种的挑战组（72.5％）和感染治疗组（56.3％）的肌肉幼虫均显着减少，其中8-OHdG和CD4 +和CD8 +细胞减少。而且，确定了感染治疗组中GATA-3的中等表达，并且在接种疫苗攻击的组中只有极少表达或没有表达。可以得出结论，用辐射减弱的幼虫进行疫苗接种可引发针对攻击性感染的免疫保护。另外，用石榴具有抗感染的治疗作用。然而，辐照疫苗取得了更好的效果，因此石榴可以用作接种疫苗的佐剂，以提供全面保护。