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Inhibitory effect of Capsicum chinense and Piper nigrum fruits, capsaicin and piperine on aflatoxins production in Aspergillus parasiticus by downregulating the expression of aflD, aflM, aflR, and aflS genes of aflatoxins biosynthetic pathway.
Journal of Environmental Science and Health, Part B ( IF 2 ) Pub Date : 2020-07-11 , DOI: 10.1080/03601234.2020.1787758
Génesis V Buitimea-Cantúa 1, 2 , Nydia E Buitimea-Cantúa 1 , María Del Refugio Rocha-Pizaña 3 , Alejandro Hernández-Morales 4 , Elisa Magaña-Barajas 5 , Jorge Molina-Torres 2
Affiliation  

Aflatoxins produced by Aspergillus parasiticus are toxic and carcinogenic metabolites. The biosynthesis of this mycotoxins is a complex process and involves at least 30 genes clustered within an approximately 82 kB gene cluster. In the present study, the effect of Capsicum chinense and Piper nigrum fruits on Aspergillus parasiticus growth and aflatoxin production were studied in relation to the expression of aflD, aflM, aflR, and aflS four; key genes of aflatoxins biosynthesis pathway. GC-EIMS analysis identified capsaicin (66,107 µg g−1) and piperine (1,138 µg g−1) as the most abundant compounds in C. chinense and P. nigrum fruits, respectively. The antifungal and anti-aflatoxigenic assays showed that C. chinense, P. nigrum, capsaicin, and piperine inhibited A. parasiticus growth and aflatoxins production in a dose-dependent manner. The piperine at 300 µg mL−1 produced higher radial growth inhibition (89%) and aflatoxin production inhibition (69%). The expression of aflatoxin biosynthetic genes was evaluated by quantitative real-time PCR (qRT-PCR) and revealed that aflatoxin inhibition occurring via downregulating the aflS and aflR, and subsequently aflD and aflM genes. These results will improve our understanding of the mechanism of aflatoxin regulation by C. chinense, P. nigrum, capsaicin, and piperine, and provides a reference for further study.



中文翻译:

通过下调黄曲霉毒素生物合成途径的aflD,aflM,aflR和aflS基因的表达,抑制辣椒和黑胡椒果实,辣椒素和胡椒碱对寄生曲霉中黄曲霉毒素产生的抑制作用。

寄生曲霉产生的黄曲霉毒素是有毒和致癌的代谢产物。这种霉菌毒素的生物合成是一个复杂的过程,涉及至少30个基因,这些基因聚集在大约82 kB基因簇中。在本研究中,研究了辣椒黑胡椒果实对寄生曲霉生长和黄曲霉毒素产生的影响,涉及afl D,afl M,afl R和afl S 4的表达。黄曲霉毒素生物合成途径的关键基因。GC-EIMS分析确定了辣椒素(66,107 µg g -1)和胡椒碱(1,138 µg g -1))分别是chin。chinenseP. nigrum果实中最丰富的化合物。抗真菌和抗黄曲霉毒素测定表明,C。chinense,P。nigrum,辣椒素和胡椒碱以剂量依赖的方式抑制了寄生曲霉的生长和黄曲霉毒素的产生。300 µg mL -1的胡椒碱产生更高的径向生长抑制(89%)和黄曲霉毒素产生抑制(69%)。通过定量实时PCR(qRT-PCR)评估了黄曲霉毒素生物合成基因的表达,并揭示了黄曲霉毒素的抑制是通过下调afl S和afl R,然后下调afl D和afl发生的。M个基因。这些结果将加深我们对中华C,黑僵菌,辣椒素和胡椒碱调节黄曲霉毒素机制的了解,并为进一步研究提供参考。

更新日期:2020-09-03
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