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Glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1, a potential target of miR-296, facilitates proliferation and migration of rectal cancer cells.
Bioscience, Biotechnology, and Biochemistry ( IF 1.6 ) Pub Date : 2020-07-11 , DOI: 10.1080/09168451.2020.1792267
Huan Ma 1 , Xianyu Zhang 1 , Na Li 1 , Xiurong Lu 1 , Yulei Wei 1 , Na Yuan 1 , Guiying Tian 1 , Shuguang Li 2
Affiliation  

The purpose of our article was to probe the influence of GRINA on rectal cancer and how GRINA is regulated in rectal cancer. Based on the public data, we found that GRINA was highly expressed in rectal cancer tissues and related to worse prognosis in rectal cancer patients. MiR-296 was predicted as an upstream regulatory miRNA of GRINA, which was further verified by dual-luciferase reporter assay. Moreover, we revealed that up-regulation/down-regulation of GRINA facilitated/suppressed SW1463/SW837 cell proliferation, migration, and invasion. Rescue assays indicated that the facilitating impact of GRINA on SW1463 cell proliferation and motility was abolished by miR-296 over-expression whilst the suppressing influence of GRINA on SW837 cell proliferation, migration, and invasion was reversed by miR-296 depletion. These consequences indicated that GRINA, which might be regulated by miR-296, acted stimulative important impact on rectal cancer cells, insinuating that GRINA might be a novel potential target for rectal cancer therapy.



中文翻译:

谷氨酸受体,离子型,N-甲基D-天冬氨酸相关蛋白1(miR-296的潜在靶标)促进直肠癌细胞的增殖和迁移。

本文的目的是探讨GRINA对直肠癌的影响以及GRINA在直肠癌中的调控方式。根据公开数据,我们发现GRINA在直肠癌组织中高表达,并且与直肠癌患者的预后较差有关。MiR-296被预测为GRINA的上游调控miRNA,已通过双荧光素酶报告基因检测进一步证实。此外,我们发现GRINA的上调/下调促进/抑制了SW1463 / SW837细胞的增殖,迁移和侵袭。救援试验表明,miR-296过表达消除了GRINA对SW1463细胞增殖和运动的促进作用,而miR-296消耗则逆转了GRINA对SW837细胞增殖,迁移和侵袭的抑制作用。

更新日期:2020-09-08
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