The sipunculid worm Sipunculus nudus is a widely distributed, commercially important marine mollusc. Oogenesis and sex determination/differentiation are important steps in S. nudus reproduction. However, the molecular mechanisms of oogenesis and sex determination/differentiation in S. nudus are unclear. We successfully generated a high‐quality de novo assembled and annotated transcriptome of oocytes from coelomic fluid (OC), oocytes from nephridium (ON) and spermatozeugmata from coelomic fluid (SC) of S. nudus using the Illumina sequencing platform. There were 17,720 and 17,030 differentially expressed genes (DEGs) in OC vs. ON and OC vs. SC respectively. KEGG pathway analysis showed that the DEGs in these two groups were enriched in ribosome, thyroid hormone synthesis, apelin signalling pathway and cellular senescence. Critical genes that play essential roles in sex determination/differentiation were found, including foxl2, fem‐1, dmrt1, ftz‐f1 and sox9. This may largely contribute to the understanding of the molecular mechanisms of oogenesis and sex determination/differentiation in S. nudus and laid a foundation for further in‐depth studies.

中文翻译：

---

鉴定天牛雌雄配子差异表达的基因

sipunculid蠕虫Sipunculus nudus是一种分布广泛，具有重要商业意义的海洋软体动物。卵子发生和性别决定/分化是重要的步骤S.星虫再现。然而，尚不清楚葡萄球菌的卵子发生和性别决定/分化的分子机制。我们成功地从腔积液（OC）中产生了高质量的从头组装和注释的卵母细胞转录组，从肾上腺液（SC）中产生了来自肾上腺（ON）的卵母细胞和来自精液的精子。使用Illumina测序平台。在OC对ON和OC对SC中分别有17,720和17,030个差异表达基因（DEG）。KEGG通路分析表明，这两组DEGs富含核糖体，甲状腺激素合成，apelin信号通路和细胞衰老。发现了在性别决定/分化中起关键作用的关键基因，包括foxl2，fem-1，dmrt1，ftz-f1和sox9。这可以大大有助于在卵子发生和性别决定/分化的分子机制的了解S.星虫奠定了进一步的深入研究奠定了基础。