当前位置: X-MOL 学术Biomed. Chromatogr. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Determination of the immunostimulatory drug-glucosoaminyl-muramyl-dipeptide-in human plasma using HPLC-MS/MS and its application to a pharmacokinetic study.
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2020-07-11 , DOI: 10.1002/bmc.4948
Natalia E Moskaleva 1 , Pavel A Markin 1, 2 , Roman M Kuznetsov 1 , Tatiana M Andronova 3 , Svetlana A Appolonova 1
Affiliation  

GMDP (glucosoaminyl‐muramyl‐dipeptide), a synthetic analog of the peptidoglycan fragment of the bacterial cell wall, is an active component of the immunomodulatory drug Licopid. But the pharmacokinetic parameters of GMDP in humans after oral administration have not been investigated yet. The present study aimed at developing and validating a sensitive LC–MS/MS method for the analysis of GMDP in human plasma. The sample was prepared by solid‐phase extraction using Strata‐X 33 μm polymeric reversed‐phase 60 mg/3 mL cartridges Phenomenex (Torrance, CA, USA). The analytes were separated using an Acquity UPLC BEN C18 column, 1.7 μm 2.1 × 50 mm Waters (Milford, USA). GMDP and internal standard growth hormone releasing peptide‐2 (pralmorelin) were ionized in positive electrospray ionization mode and detected in multiple reaction monitoring mode. The developed method was validated within a linear range of 50–3000 pg/mL for GMDP. Accuracy for all analytes, given as the deviation between the nominal and measured concentration and assay variability , ranged from 1.61 to 3.02% and from 0.89 to 1.79%, respectively, for both within‐ and between‐run variabilities. The developed and validated HPLC–MS/MS method was successfully used to obtain the plasma pharmacokinetic profiles of GMDP distribution in human plasma.

中文翻译:

使用HPLC-MS / MS测定人体血浆中的免疫刺激药物-葡萄糖胺基-村m基-二肽-及其在药代动力学研究中的应用。

GMDP(葡萄糖胺基-村酰胺基-二肽)是细菌细胞壁肽聚糖片段的合成类似物,是免疫调节药物Licopid的活性成分。但是尚未研究口服后GMDP在人体内的药代动力学参数。本研究旨在开发和验证用于分析人血浆中GMDP的灵敏LC-MS / MS方法。使用Strata-X 33μm聚合物反相60 mg / 3 mL色谱柱Phenomenex(Torrance,CA,USA)通过固相萃取制备样品。使用1.7μm2.1×50 mm Waters(Milford,USA)的Acquity UPLC BEN C18色谱柱分离分析物。GMDP和内标生长激素释放肽2(普尔莫林)在正电喷雾电离模式下被电离,并在多反应监测模式下进行检测。对于GMDP,已开发的方法在50-3000 pg / mL的线性范围内得到验证。批内和批间变异性的所有分析物的准确度(标称浓度与测量浓度和测定变异性之间的偏差)分别为1.61至3.02%和0.89至1.79%。已开发和验证的HPLC-MS / MS方法已成功用于获得GMDP在人血浆中分布的血浆药代动力学特征。
更新日期:2020-07-11
down
wechat
bug