The majority of oligosaccharides used as prebiotics typically consist of a combination of 3 kinds of neutral monosaccharides, d-glucose, d-galactose, and d-fructose. In this context, we aimed to generate new types of prebiotic oligosaccharides containing other monosaccharides, and to date have synthesized various oligosaccharides containing an amino sugar, uronic acid, and their derivatives. In this study, we investigated the effects of 4 kinds of sucrose (Suc) analog disaccharides containing d-glucosamine, N-acetyl-d-glucosamine, d-glucuronic acid, or d-glucuronamide as constituent monosaccharides, on the growth of 8 species of bifidobacteria and 3 species of lactobacilli isolated from the human intestine. The results of these experiments were compared with those obtained from identical experiments using Suc. We confirmed that all bacterial strains could utilize Suc as a nutrient source for growth; in contrast, only specific species of bifidobacteria showed growth with Suc analog disaccharides. When oligosaccharides are utilized as a nutrient source by bacteria, they are often broken down into monosaccharides or their derivatives by cellular enzymes before entering the intracellular glycolytic pathway. Therefore, to clarify the above phenomenon involved in the growth of bifidobacteria using Suc analog disaccharides, we investigated the cellular glycosidases of 3 strains of bifidobacteria shown to be capable or incapable of growth in the presence of these disaccharides. As the result, it was confirmed that the strains capable of growth using Suc analog disaccharides show greater productivity of glycosidases that degrade these disaccharides than strains not capable of growth; however, we have not identified the enzymes here.

用作益生元的大多数寡糖通常由d-葡萄糖，d-半乳糖和d-果糖3种中性单糖的组合组成。在这种情况下，我们的目标是产生包含其他单糖的新型益生元低聚糖，迄今为止，已经合成了各种包含氨基糖，糖醛酸及其衍生物的低聚糖。在这项研究中，我们调查了4种蔗糖（的Suc）包含二糖类似物的影响d -葡糖胺，Ñ乙酰基d -葡糖胺，d -glucuronic酸，或d-葡萄糖醛酸酰胺作为构成单糖，对从人肠分离的8种双歧杆菌和3种乳杆菌的生长具有促进作用。将这些实验的结果与使用Suc从相同实验中获得的结果进行比较。我们证实，所有细菌菌株都可以利用Suc作为生长的营养源。相反，只有双歧杆菌的特定种类显示与Suc类似物二糖一起生长。当细菌将寡糖用作营养源时，它们通常会在进入细胞内糖酵解途径之前被细胞酶分解为单糖或其衍生物。因此，为澄清上述使用Suc类似物二糖参与双歧杆菌生长的现象，我们研究了3种双歧杆菌菌株的细胞糖苷酶，这些菌株在这些二糖存在下能够或不能生长。结果，证实了能够使用Suc类似物二糖生长的菌株比不能生长的菌株显示出降解这些二糖的糖苷酶的生产力更高。但是，我们尚未在此处鉴定酶。