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A preliminary phylogenetic analysis of ribbed-pine-borer (Rhagium inquisitor) based on mitochondrial COI sequences
Journal of Asia-Pacific Entomology ( IF 1.5 ) Pub Date : 2020-07-11 , DOI: 10.1016/j.aspen.2020.06.008
Yunus Emre Çakmak , Havva Kübra Soydabaş-Ayoub , Fevzi Uçkan

The increased volume of intercontinental-trade had facilitated the distribution of species, including invaders. Wooden packaging materials, goods and timber are being transported via international ports in East of the Marmara Basin, where has vast forests. That forests provide an appropriate-mediator for the introduction and invasion of wood-boring insects, which are the most common group being transported between continents. One of them is Rhagium inquisitor, which has a pivotal role in wood decay, nevertheless, it is detrimental for industrial wood.

We analyzed the 654 bp length of cytochrome c oxidase I (COI) gene, to determine native and introduced haplotypes of the ribbed-pine-borer, to investigate their phylogenetic relationships and possibly cryptic speciation of the morphospecies.

It was found out that sixteen haplotypes were divided into five haplogroups. The groups were assembled on two main lineages: Eurasian and American. The genetic distance between the American (Groups 1–3), Asian (Group-4) and European (Group-5) clusters ranged from 3 to 10%; between haplotypes ranged from 0.2 to 10.9%. The distances between the specimens of the Marmara Basin (H9-12, Group-4) were lower compared to H16, which nested in Group-5. That haplotype was sampled on imported timber and was found out that it is shared between France, Germany, Italy, and Austria. Maximum Likelihood, Neighbor-Joining and Bayesian Inference trees showed the same topologies, and Median-Joining network also supported them.

Contrary to the usual, our findings point out that some species might have been introduced from Europe to Asia. Furthermore, the high genetic diversity of the bark-runner between continents could be a sign of cryptic-allopatric speciation.



中文翻译:

基于线粒体COI序列的带肋松borRhagium inquisitor)的初步系统发育分析

洲际贸易量的增加促进了包括入侵者在内的物种的分布。木制包装材料,货物和木材正通过马尔马拉盆地以东的国际港口运输,那里有广阔的森林。森林为介导无聊的木材昆虫提供了适当的媒介,这是在大陆之间运输的最常见的一种。其中之一是Rhagium inquisitor,它在木材腐烂中起着举足轻重的作用,但是,它对工业木材有害。

我们分析了细胞色素C氧化酶I(COI)基因的654 bp长度,以确定带肋松-的天然和引入的单倍型,以研究它们的系统发生关系和可能的形态学物种。

结果发现,有十六种单倍型被分为五个单倍型。这些团体聚集在两个主要血统:欧亚和美洲。美洲(第1-3组),亚洲(第4组)和欧洲(第5组)集群之间的遗传距离范围为3%至10%。单倍型之间的差异为0.2%至10.9%。与嵌套在第5组中的H16相比,马尔马拉盆地(H9-12,第4组)的标本之间的距离要短。该单倍型是在进口木材上取样的,发现它在法国,德国,意大利和奥地利之间共享。最大似然树,邻居加入树和贝叶斯推理树显示了相同的拓扑,中值加入网络也支持它们。

与通常情况相反,我们的发现指出某些物种可能已从欧洲引入亚洲。此外,各大洲之间的树皮运行者的高遗传多样性可能是隐密异特异物种形成的迹象。

更新日期:2020-07-11
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