Diagnostic Microbiology and Infectious Disease ( IF 2.9 ) Pub Date : 2020-07-12 , DOI: 10.1016/j.diagmicrobio.2020.115131 Augusto César Parreiras de Jesus 1 , Ana Laura Grossi de Oliveira 1 , Nathalia Sernizon Guimarães 1 , Leonel Mendoza 2 , Ricardo Toshio Fujiwara 3 , Cristiane Alves da Silva Menezes 4 , Raquel Virginia Rocha Vilela 5
Some species of the fungus Sporothrix cause a chronic granulomatous infection in humans and animals called sporotrichosis. In the last decades, some research into serological tests has been carried out by different groups for the rapid detection of this infection. We performed a systematic review of the literature with meta-analysis to evaluate studies using Sporothrix spp. antigens and to evaluate their accuracy for sporotrichosis diagnostic. We searched Scopus, MEDLINE, Web of Science, GALE, Technology Research Database, DOA, Elsevier, SciELO, and Google Scholar Databases. The united results of sensitivity, specificity, positive and negative likelihood ratios, and diagnostic odds ratio with their corresponding 95% confidence intervals (CI) were assessed. A total of 15 assays from 8 studies using 7 different serological methods and 8 different antigens were analyzed. The studies were performed in the USA, Brazil, and Venezuela from 1973 until 2015 and presented good quality. A high heterogeneity for sensitivity [I2 = 90.7%; 87% CI = (84–89), P < 0.001] and specificity [I2 = 89.2%; 93% CI = (92–95), P < 0.001] was observed. The performance of diagnostic tests was 0.93. Enzyme-linked immunosorbent assay was the main tool used, and the ConA-binding fraction antigen of the strain 1099-18 appears as a promising diagnostic biomarker candidate.
中文翻译:
使用孢粉菌种抗原进行血清学检测以准确诊断孢子囊病:一项荟萃分析。
某些种类的孢子霉菌会在人类和动物中引起慢性肉芽肿性感染,称为孢子虫病。在过去的几十年中,为了快速检测这种感染,不同的研究小组对血清学检测进行了一些研究。我们通过荟萃分析对文献进行了系统的综述,以评估使用孢子丝菌的研究。spp。抗原,并评估其准确性,用于孢子虫病的诊断。我们搜索了Scopus,MEDLINE,Web of Science,GALE,技术研究数据库,DOA,Elsevier,SciELO和Google Scholar数据库。评估了敏感性,特异性,阳性和阴性可能性比以及诊断比值比及其相应的95%置信区间(CI)的统一结果。使用8种不同的血清学方法和8种不同的抗原对来自8项研究的15种测定法进行了分析。这项研究于1973年至2015年在美国,巴西和委内瑞拉进行,并且质量良好。灵敏度的高度异质性[ I 2 = 90.7%;87%CI =(84–89),P <0.001]和特异性[ I 2 = 89.2%; 观察到93%CI =(92-95),P <0.001]。诊断测试的性能为0.93。酶联免疫吸附测定是使用的主要工具,菌株1099-18的ConA结合级分抗原似乎是有前途的诊断生物标志物候选物。