Introduction

SARS-CoV-2 seroconversion is important for epidemiological studies as well as contact tracing.

Material and methods

The antibody response against SARS-CoV-2 was examined in 111 patients with a positive qRT-PCR. Seroconversion was assessed using the Elecsys from Roche, the Liaison S1/S2 IgG from Diasorin, the IgG and IgA from Euroimmun, as well as the VIDAS IgG and IgM. Specificity was estimated based on the measurement of SARS-CoV-2 antibodies in 96 residual samples collected during a non-pandemic period.

Results

The highest overall sensitivity for detecting seroconversion was obtained using the Elecsys (81.1%), the Euroimmun with a combined detection of IgG/IgA (86.5%), and the VIDAS with a simultaneous measurement of IgG/IgM (78.4%).The Elecsys and the VIDAS IgG/IgM demonstrated a specificity as well as a positive predictive value of 100%.

Conclusions

The Elecsys and the VIDAS methods with a combination of IgG/IgM measurement demonstrated a high sensitivity with no false positive results.

中文翻译：

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监测SARS-CoV-2感染后的抗体应答：4种自动免疫测定的诊断效率。

介绍

SARS-CoV-2血清转化对于流行病学研究以及接触者追踪都很重要。

材料与方法

在111例qRT-PCR阳性的患者中检查了针对SARS-CoV-2的抗体反应。使用Roche的Elecsys，Diasorin的Liaison S1 / S2 IgG，Euroimmun的IgG和IgA以及VIDAS IgG和IgM评估血清转化。基于在非大流行时期收集的96份残留样品中SARS-CoV-2抗体的测量值来评估特异性。

结果

使用Elecsys（81.1％），同时结合检测IgG / IgA的Euroimmun（86.5％）和同时检测IgG / IgM的VIDAS（78.4％）获得了最高的检测血清转化的总灵敏度。 VIDAS IgG / IgM表现出特异性，并具有100％的阳性预测值。

结论

Elecsys和VIDAS方法结合IgG / IgM测量显示出高灵敏度，没有假阳性结果。