Microbial cell factories offer great opportunities for the feasible biosynthesis of various value-added products from renewable resources. In recent years, protein engineering has served as a powerful approach for generating enzymes or proteins with desirable properties. As an effective toolbox, many efforts have been employed into protein engineering for the improvement of biological networks by enhancing, regulating, expanding, and innovating metabolic pathways. In combination with traditional metabolic engineering strategies, protein engineering has demonstrated tremendous promise in facilitating the production of many bio-based products. In this review, we present recent trends and strategies available for protein engineering, and highlight the successful interplay between protein and metabolic engineering in boosting the production of target compounds using diverse microbial cell factories. The perspectives for protein design in metabolic engineering are also briefly discussed.

微生物细胞工厂为利用可再生资源进行各种增值产品的可行生物合成提供了巨大的机会。近年来，蛋白质工程已成为产生具有所需特性的酶或蛋白质的有力方法。作为有效的工具箱，已经通过增强，调节，扩展和创新代谢途径，对蛋白质工程进行了许多努力来改善生物网络。与传统的代谢工程策略相结合，蛋白质工程在促进许多生物基产品的生产方面显示出巨大的希望。在这篇评论中，我们介绍了蛋白质工程的最新趋势和策略，并强调了蛋白质和代谢工程学在利用多种微生物细胞工厂提高目标化合物产量方面的成功相互作用。还简要讨论了代谢工程中蛋白质设计的观点。