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Influence of RNA interference-mediated reduction of Or11 on the expression of transcription factor Kr-h1 in Apis mellifera drones
Insectes Sociaux ( IF 1.3 ) Pub Date : 2020-07-11 , DOI: 10.1007/s00040-020-00775-4
J. F. Liu , X. J. Wan , Z. L. Wang , X. J. He , Z. J. Zeng

9-Oxo-2-decenoic acid (9-ODA, the predominant component of honeybee queen mandibular pheromones) acts as a sex pheromone attracting drones during mating flights in midair. Odorant receptor 11 (Or11), which is located on the membrane of antennal olfaction receptor neurons in bees, can specifically recognize 9-ODA. At present, the molecular pathway of honeybee drones responding to 9-ODA is still unclear. Studies have demonstrated that 9-ODA could downregulate the expression of Krüppel-homolog1 (Kr-h1, a transcription factor related to the regulation of reproduction and division of labor mediated by juvenile hormone) gene in the mushroom of honeybee brain. We speculate that Kr-h1 may be the downstream gene of Or11, which is involved in the pathway of drones responding to 9-ODA. Therefore, we analyzed the influence of 9-ODA on the expression of Or11 and Kr-h1 in the antennae of sexually immature (4 days) and mature (14 days) male honeybees (Apis mellifera) by quantitative polymerase chain reaction (qPCR). The results demonstrated that 9-ODA significantly downregulated the expression of Or11 and Kr-h1 in the antennae of sexually immature and mature drones. Additionally, siRNA-Or11 was injected into the antennae and brain tissues of 8-day-old drone pupae, and the expression patterns of Or11, Kr-h1 and Broad-Complex (Br–c, downstream gene of Kr-h1) were determined by qPCR at 72 h. The RNAi-induced knockdown of Or11 significantly decreased the expression of Or11, Kr-h1 and Br–c in the antennae and brains of drones. This study suggests that the transcription factor Kr-h1 is downstream of Or11, Kr-h1, which may play an important role in the signal transduction process of drones responding to 9-ODA.

中文翻译:

RNA干扰介导Or11减少对蜜蜂雄蜂转录因子Kr-h1表达的影响

9-Oxo-2-癸烯酸(9-ODA,蜜蜂女王下颌信息素的主要成分)作为一种性信息素,在空中交配飞行期间吸引雄蜂。气味受体 11 (Or11) 位于蜜蜂触角嗅觉受体神经元的膜上,可以特异性识别 9-ODA。目前,蜜蜂雄蜂响应9-ODA的分子途径尚不清楚。研究表明,9-ODA可以下调蜜蜂脑蘑菇中Krüppel-homolog1(Kr-h1,一种与保幼激素介导的生殖和分工调节相关的转录因子)基因的表达。我们推测 Kr-h1 可能是 Or11 的下游基因,该基因参与了无人机响应 9-ODA 的途径。所以,我们通过定量聚合酶链反应 (qPCR) 分析了 9-ODA 对性未成熟(4 天)和成熟(14 天)雄性蜜蜂(Apis mellifera)触角中 Or11 和 Kr-h1 表达的影响。结果表明,9-ODA显着下调性未成熟和成熟雄蜂触角中Or11和Kr-h1的表达。此外,将siRNA-Or11注射到8日龄雄蜂蛹的触角和脑组织中,确定Or11、Kr-h1和Broad-Complex(Br-c,Kr-h1的下游基因)的表达模式通过 qPCR 在 72 小时。RNAi 诱导的 Or11 敲低显着降低了无人机触角和大脑中 Or11、Kr-h1 和 Br-c 的表达。该研究表明转录因子 Kr-h1 位于 Or11、Kr-h1、
更新日期:2020-07-11
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