The effect of growth regulators was investigated to enhance biomass growth and apigenin production. Murashige and Skoog medium supplemented with 2,4-D (1.5 mg l⁻¹) and Kinetin (0.5 mg l⁻¹) showed the highest callus induction rate (100%), fresh weight, apigenin (0.82%) and apigenin-7-glucoside (1.57%) contents. Cell suspension culture was established, and the optimum subculture time was found to 13–15 days. SMF induced cell leaching and oxidative stress in all treated cells by an increase in H₂O₂ content and more stimulation of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) enzymes activities. Total phenolic, flavonoid and DPPH activity increased in cells treated to SMF, and the maximum content of apigenin (1.3%) and apigenin-7-glucoside (2.1%) were identified in cell treated to 4 mT. These results provided an effective method for the regulation of flavonoid biosynthesis in M. chamomilla cell suspension culture, and the use of SMF as a tool for the induction of apigenin production.

研究了生长调节剂的作用，以增强生物量的生长和芹菜素的生产。补充有2,4-D（1.5 mg l ^-1）和Kinetin（0.5 mg l ^-1）的Murashige和Skoog培养基显示出最高的愈伤组织诱导率（100％），鲜重，芹菜素（0.82％）和芹菜素7 -葡萄糖苷（1.57％）含量。建立了细胞悬浮培养，发现最佳的亚培养时间为13-15天。SMF通过增加H ₂ O ₂诱导所有处理过的细胞浸出和氧化应激含量和更多刺激超氧化物歧化酶（SOD），过氧化氢酶（CAT）和过氧化物酶（POX）酶的活性。用SMF处理的细胞中总酚，类黄酮和DPPH活性增加，在处理至4 mT的细胞中，芹菜素的最大含量（1.3％）和芹菜素-7-葡萄糖苷（2.1％）被确定。这些结果提供了一种有效的方法，用于调节沙门氏菌细胞悬浮培养物中的类黄酮生物合成，以及将SMF用作诱导芹菜素生产的工具。