Plants respond to the environmental perturbations by triggering the dynamic changes within the transcriptome. The assessment of these oscillations within the transcriptome would offer insights into the ecological adaptation of the plants. We evaluated how the transcriptome of Taxus contorta swings under natural conditions to elucidate its adaptive response. Thus, our study provides new insights into the performance of T. contorta under a changing environment during different seasons. The abundance estimation using the RNAseq approach revealed 6727 differentially expressed genes. Comprehensive reprogramming was observed in Taxol biosynthesis, maintenance of redox homeostasis, and generation of effective shield to UV-B, high light intensity, and temperature. Besides differential expression, the alternative splicing (AS) and single nucleotide variations (SNVs) also confer flexibility to the transcriptome of T. contorta. 1936 differentially expressing transcripts were also found to exhibit Differential Exon Usage (DEU) as well as differential SNVs. LC–MS-based untargeted metabolic analysis revealed 7774 ion features, among which around 334 putatively identified metabolites were differentially regulated. Our results showed that the swing and the oscillations of the transcriptome and metabolome of T. contorta ensure adaptability and better survival under changing environment. In addition, varying patterns of AS and SNVs compliment the adaptation provided by differential expression.

植物通过触发转录组内的动态变化来响应环境扰动。转录组中这些振荡的评估将提供对植物生态适应性的见解。我们评估了红豆杉转录组在自然条件下如何摆动以阐明其适应性反应。因此，我们的研究为T. contorta的性能提供了新的见解。在不同季节变化的环境中。使用RNAseq方法进行的丰度估计揭示了6727个差异表达的基因。在紫杉醇的生物合成，氧化还原稳态的维持以及对UV-B的有效屏蔽，高光强度和温度的产生中，观察到了全面的重新编程。除了差异表达外，可变剪接（AS）和单核苷酸变异（SNV）还赋予了T. contorta转录组灵活性。。1936年，差异表达的转录本也被发现具有差异外显子用法（DEU）和差异SNV。基于LC-MS的非靶向代谢分析揭示了7774个离子特征，其中约334个推定识别的代谢物受到差异调节。我们的研究结果表明，扭曲的转录组和代谢组的摆动和振荡确保了在不断变化的环境下的适应性和更好的生存能力。另外，AS和SNV的变化模式补充了差异表达提供的适应性。