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Up-regulation of GhPAP1A results in moderate anthocyanin accumulation and pigmentation in sub-red cotton.
Molecular Genetics and Genomics ( IF 3.1 ) Pub Date : 2020-07-10 , DOI: 10.1007/s00438-020-01708-3
Aimin Liang 1 , Jia Zhao 1 , Xin Li 1 , Fulin Yan 1 , Zhong Chen 1 , Xusheng Chen 2 , Yi Wang 1 , Yaohua Li 1 , Chuannan Wang 1 , Yuehua Xiao 1
Affiliation  

Anthocyanins are a group of important secondary metabolites, functioning as colorant in plant organs as well as protective agents against several stresses. Sub-red plant (Rs) cottons, accumulating moderate level of anthocyanins in shoots, had increased photosynthesis efficiency compared to green- (GL) and red-plant (R1) cottons. The present work aimed to clarify the molecular base of anthocyanin regulation in Rs cotton. It was found that GhPAP1A was significantly up-regulated in Rs plants compared to GL cottons, but its expression level is lower than that of GhPAP1D in R1 plants. Virus induced gene silencing of GhPAP1s inhibited the red pigmentation in Rs plants. Comparative cloning revealed a 50-bp tandem repeat in the promoter of GhPAP1A in Rs cotton, which showed stronger activity to drive the expression of downstream genes in petals. Considered that the coding sequence of GhPAP1As from Rs and GL cottons had similar functions to promote anthocyanin biosynthesis in transgenic tobaccos, we attributed moderate anthocyanin accumulation in Rs cotton to increased transcription of GhPAP1A, resulted from varied promoter structure. Our works suggested GhPAP1s as useful tool to manipulate anthocyanin level and several breeding targets, including herbivore- and pathogen- resistance, high photosynthesis efficiency and colored fibers.



中文翻译:

GhPAP1A的上调导致亚红棉中花色苷的适度积累和色素沉着。

花青素是一组重要的次级代谢产物,在植物器官中起着着色剂的作用,并能抵抗多种胁迫。与绿色(GL)和红色植物(R1)棉花相比,亚红色植物(Rs)棉花在芽中积累适度的花色苷水平,具有更高的光合作用效率。本工作旨在阐明Rs棉花中花色苷调节的分子基础。已经发现,与GL棉相比,Rs植物中GhPAP1A显着上调,但在R1植物中其表达水平低于GhPAP1D。病毒诱导的GhPAP1s基因沉默抑制了Rs植物中的红色色素沉着。比较克隆显示,Rs棉花的GhPAP1A启动子中有一个50 bp的串联重复序列,这显示出较强的活性来驱动花瓣中下游基因的表达。考虑到来自Rs和GL棉花的GhPAP1A的编码序列具有促进转基因烟草中花色苷生物合成的相似功能,我们将Rs棉花中适度的花色苷积累归因于GhPAP1A的转录增加,这是由于启动子结构不同所致。我们的工作表明,GhPAP1s是控制花色苷水平和几个育种目标的有用工具,包括抗草食动物和病原体,高光合作用效率和有色纤维。

更新日期:2020-07-13
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