Long non-coding RNAs (lncRNAs) can not only regulate gene transcription and translation, but also participate in the development of central nervous system diseases as epigenetic modification factors. However, their functional significance in atherosclerosis-induced ischemic stroke (AIIS) is unclear. The study aimed to screen out differentially expressed lncRNAs (delncRNAs), and to elucidate their potential regulatory mechanisms in the pathophysiology of AIIS. Based on the clinicopathological features and clinical images, we screened out 10 patients with AIIS and recruited 10 healthy volunteers. Then we used microarray to detect the whole blood RNA of subjects, and explored the biological functions of delncRNAs by GO and KEGG analysis. After further analyzing the delncRNAs of THP-1 stimulated with ox-LDL, selective lncRNAs were screened and a corresponding lncRNA–mRNA interaction network was constructed through co-expression analysis. We yielded 180 delncRNAs (44 up-regulated and 136 down-regulated) and 218 demRNAs (45 up-regulated and 173 down-regulated). Lnc-SCARNA8 and lnc-SNRPN-2 are the most significant elevated and decreased lncRNA in AIIS, respectively. The delncRNAs may play a significant role in ubiquitination-mediated protein degradation signaling pathways. According to lncRNA–mRNA network, the expression of vacuolar protein sorting 13 homolog B (VPS13B) and biliverdin reductase B (BLVRB) were significantly regulated. Our findings suggest that the ubiquitinated proteasome pathway, VPS13B and BLVRB may play a fundamental role in the pathological process of AIIS.

长链非编码RNA（lncRNA）不仅可以调节基因的转录和翻译，还可以作为表观遗传修饰因子参与中枢神经系统疾病的发展。然而，它们在动脉粥样硬化性缺血性卒中 (AIIS) 中的功能意义尚不清楚。该研究旨在筛选出差异表达的lncRNA（delncRNA），并阐明其在AIIS病理生理学中的潜在调控机制。根据临床病理特征和临床影像，筛选出10名AIIS患者，招募10名健康志愿者。然后我们使用微阵列检测受试者的全血RNA，并通过GO和KEGG分析探索delncRNA的生物学功能。在进一步分析了 ox-LDL 刺激的 THP-1 的 delncRNAs 后，筛选出选择性lncRNA，并通过共表达分析构建了相应的lncRNA-mRNA相互作用网络。我们产生了 180 个 delncRNA（44 个上调和 136 个下调）和 218 个 demRNA（45 个上调和 173 个下调）。Lnc-SCARNA8 和 lnc-SNRPN-2 分别是 AIIS 中最显着升高和降低的 lncRNA。delncRNA 可能在泛素化介导的蛋白质降解信号通路中发挥重要作用。根据 lncRNA-mRNA 网络，液泡蛋白分选 13 同源物 B (VPS13B) 和胆绿素还原酶 B (BLVRB) 的表达受到显着调节。我们的研究结果表明，泛素化的蛋白酶体途径、VPS13B 和 BLVRB 可能在 AIIS 的病理过程中起基础性作用。