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Guiding neural extensions of PC12 cells on carbon nanotube tracks dielectrophoretically formed in poly(ethylene glycol) dimethacrylate
RSC Advances ( IF 3.9 ) Pub Date : 2020-7-10 , DOI: 10.1039/d0ra04496b
Fikri Seven 1 , Tansu Gölcez 1 , Ziyşan Buse Yaralı 2 , Günnur Onak 2 , Ozan Karaman 3 , Mustafa Şen 3
Affiliation  

The PC12 cell line has been widely used as an in vitro model for studying neuronal differentiation and identifying the factors affecting the process. It has the ability to differentiate in the presence of nerve growth factor (NGF), resulting in neural extensions called dendrites and axons. In this study, first the impact of randomly distributed multi-walled carbon nanotubes (MWCNTs) in poly(ethylene glycol) dimethacrylate (PEGDMA) on PC12 cell differentiation was investigated in terms of neurite length, number of neurite per cell and differentiation marker gene expression profile. Then, dielectrophoretically aligned MWCNTs in PEGDMA was used to guide and support the neuronal differentiation of PC12 cells in the presence of NGF. The method is expected to be useful in revealing the nanotopographical role in fundamental studies and understanding of nanotopographical effects for biomedical applications on nerve regeneration.

中文翻译:

在聚(乙二醇)二甲基丙烯酸酯介电泳形成的碳纳米管轨道上引导 PC12 细胞的神经延伸

PC12 细胞系已被广泛用作体外用于研究神经元分化和确定影响过程的因素的模型。它具有在存在神经生长因子 (NGF) 的情况下进行分化的能力,从而产生称为树突和轴突的神经延伸。在这项研究中,首先研究了聚(乙二醇)二甲基丙烯酸酯(PEGDMA)中随机分布的多壁碳纳米管(MWCNTs)对PC12细胞分化的影响,包括神经突长度、每个细胞的神经突数量和分化标记基因表达。轮廓。然后,在 PEGDMA 中使用介电泳排列的 MWCNTs 来指导和支持在 NGF 存在下 PC12 细胞的神经元分化。
更新日期:2020-07-10
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