An Egyptian indigenous unicellular green microalga was isolated from the coastal water of Suez Bay (N 29.92°, E 32.473°), Red Sea, Egypt. The molecular analysis based on 18S rRNA sequence showed that the gene sequence for this strain was highly similar (100% identity and 98% query cover) to different Chlorella strains isolated from different habitats. The observed morphological characters together with the molecular phylogeny assigned the isolated microalga as Chlorella sp. MF1 with accession number KX228798. This isolated strain was cultivated for estimation of its growth and biochemical composition. The mean specific growth rate (μ) was 0.273 day−1. Both the biomass productivity and the cellular lipid content increased by increasing salinity of the growth medium, recording a maximum of 6.53 gDW l−1 and 20.17%, respectively, at salinity 40.4. Fourteen fatty acids were identified. The total saturated fatty acid percentage was 54.73% with stearic (C18:0), arachidic (C20:0), and palmitic acids (C16:0) as major components, while the total unsaturated fatty acid percentage was 45.27% with linoleic acid (C18:2c) and oleic acid (C18:1) as majors. This algal strain proved to be a potential newly introduced microalga as one of the most proper options available for microalgae-based biodiesel production. The proximate analysis showed the protein content at 39.85% and carbohydrate at 23.7%, indicating its accessibility to various purposes.

中文翻译：

---

海洋绿藻Chlorella sp。的分子鉴定，生物量和生化组成。从苏伊士湾隔离的MF1。

从埃及红海苏伊士湾沿海水域（北29.92°，东32.473°）分离出埃及土著单细胞绿色微藻。基于18S rRNA序列的分子分析表明，该菌株的基因序列与从不同栖息地分离的不同小球藻菌株高度相似（100％同一性和98％查询覆盖率）。观察到的形态特征以及分子系统发育将分离的微藻命名为小球藻。MF1，登录号为KX228798。培养该分离的菌株以估计其生长和生化组成。平均比增长率（μ）为0.273天-1。生物量生产力和细胞脂质含量都通过增加生长培养基的盐度而增加，在盐度40.4时分别记录最大6.53 gDW 1-1和20.17％。鉴定出十四种脂肪酸。以硬脂酸（C18：0），花生酸（C20：0）和棕榈酸（C16：0）为主要成分的总饱和脂肪酸百分比为54.73％，而亚油酸（C18：0），棕榈酸（C16：0）的总饱和脂肪酸百分比为45.27％。 C18：2c）和油酸（C18：1）为主要成分。该藻种菌株被证明是潜在的新引入的微藻类，是基于微藻类生物柴油生产的最合适的选择之一。最近的分析显示蛋白质含量为39.85％，碳水化合物含量为23.7％，表明其可用于多种用途。2c）和油酸（C18：1）为主要成分。该藻种菌株被证明是潜在的新引入的微藻类，是基于微藻类生物柴油生产的最合适的选择之一。最近的分析显示蛋白质含量为39.85％，碳水化合物含量为23.7％，表明其可用于多种用途。2c）和油酸（C18：1）为主要成分。该藻种菌株被证明是潜在的新引入的微藻类，是基于微藻类生物柴油生产的最合适的选择之一。最近的分析显示蛋白质含量为39.85％，碳水化合物含量为23.7％，表明其可用于多种用途。