Marker gene surveys have a wide variety of applications in species identification, population genetics, and molecular epidemiology. As these methods expand to new types of organisms and additional markers beyond 16S and 18S rRNA genes, comprehensive databases are a critical requirement for proper analysis of these data. Here we present an ITS2 rDNA database for marker gene surveys of both free-living and parasitic nematode populations and the software used to build the database. This is currently the most complete and up-to-date ITS2 database for nematodes and is able to reproduce previous analysis that used a smaller database. This database is an important resource for researchers working on nematodes and also provides a tool to create ITS2 databases for any given taxonomy.

中文翻译：

---

线虫的ITS2序列数据库。

标记基因调查在物种鉴定，种群遗传学和分子流行病学中具有广泛的应用。随着这些方法扩展到新型生物体和16S和18S rRNA基因之外的其他标记，全面的数据库是正确分析这些数据的关键要求。在这里，我们介绍了一个ITS2 rDNA数据库，用于自由生活和寄生线虫种群的标记基因调查以及用于构建该数据库的软件。这是目前针对线虫的最完整，最新的ITS2数据库，并且能够复制以前使用较小数据库的分析。该数据库是从事线虫研究的重要资源，也提供了为任何给定分类法创建ITS2数据库的工具。