In vitro senescence of multipotent cells has been commonly associated with DNA damage induced by oxidative stress. These changes may vary according to the sources of production and the studied lineages, which raises questions about the effect of growing time on genetic stability. This study is aimed at evaluating the evolution of genetic stability, viability, and oxidative stress of bone marrow mesenchymal stem cells (MSCBMsu) and renal progenitor cells of the renal cortex (RPCsu) of swine (Sus scrofa domesticus) in culture passages. P2, P5, and P9 were used for MSCBMsu and P1, P2, and P3 for RPCsu obtained by thawing. The experimental groups were submitted to MTT, apoptosis and necrosis assays, comet test, and reactive substance measurements of thiobarbituric acid (TBARS), nitrite, reduced glutathione (GSH), and catalase. The MTT test curve showed a mean viability of and , respectively, for MSCBMsu and RPCsu. The percentages of MSCBMsu and RPCsu were presented, respectively, for apoptosis, an irregular and descending behavior, and necrosis, ascending and irregular. The DNA damage index showed higher intensity among the MSCBMsu in the P5 and P9 passages (). In the TBARS evaluation, there was variation among the lines of RPCsu and MSCBMsu, presenting the last most significant variations (). In the nitrite values, we identified only among the lines, in the passages P1 and P2, with the highest averages displayed by the MSCBMsu lineage (). The measurement of antioxidant system activity showed high standards, identifying differences only for GSH values, in the RPCsu lineage, in P3 (). This study suggests that the maintenance of cell culture in the long term induces lower regulation of oxidative stress, and RPCsu presents higher genetic stability and lower oxidative stress than MSCBMsu during in vitro expansion.

中文翻译：

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肾祖细胞在体外扩增过程中比间充质干细胞具有更高的遗传稳定性和更低的氧化应激。

多能细胞的体外衰老通常与氧化应激诱导的 DNA 损伤有关。这些变化可能会根据生产来源和所研究的谱系而有所不同，这引发了关于生长时间对遗传稳定性影响的问题。本研究旨在评估猪（Sus scrofa Domesticus）骨髓间充质干细胞（MSCBMsu）和肾皮质肾祖细胞（RPCsu）在培养传代中的遗传稳定性、活力和氧化应激的进化。P2、P5和P9用于MSCBMsu，P1、P2和P3用于通过解冻获得的RPCsu。实验组进行MTT、细胞凋亡和坏死测定、彗星试验以及硫代巴比妥酸（TBARS）、亚硝酸盐、还原型谷胱甘肽（GSH）和过氧化氢酶的反应物质测量。MTT 测试曲线显示平均生存力和，分别适用于 MSCBMsu 和 RPCsu。MSCBMsu 和 RPCsu 的百分比分别表示细胞凋亡、不规则和下降行为以及坏死、上升和不规则行为。P5 和 P9 代中 MSCBMsu 的 DNA 损伤指数显示出更高的强度（）。在TBARS评估中，RPCsu和MSCBMsu品系之间存在差异，呈现最后最显着的差异（）。在亚硝酸盐值中，我们仅在 P1 和 P2 代中的品系中进行鉴定，MSCBMsu 谱系显示的平均值最高（）。抗氧化系统活性的测量显示出高标准，仅在 RPCsu 谱系、P3 中识别 GSH 值的差异（）。这项研究表明，长期维持细胞培养会导致氧化应激调节降低，并且在体外扩增过程中，RPCsu 比 MSCBMsu 表现出更高的遗传稳定性和更低的氧化应激。