Rapid cryopreservation of biological specimens is the gold standard for visualizing cellular structures in their true structural context. However, current commercial cryo-fluorescence microscopes are limited to low resolutions. To fill this gap, we have developed cryoSIM, a microscope for 3D super-resolution fluorescence cryo-imaging for correlation with cryo-electron microscopy or cryo-soft X-ray tomography. We provide the full instructions for replicating the instrument mostly from off-the-shelf components and accessible, user-friendly, open-source Python control software. Therefore, cryoSIM democratizes the ability to detect molecules using super-resolution fluorescence imaging of cryopreserved specimens for correlation with their cellular ultrastructure.

中文翻译：

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CryoSIM：用于相关超微结构成像的超分辨率 3D 结构照明低温荧光显微镜

生物标本的快速冷冻保存是在真实结构背景下可视化细胞结构的黄金标准。然而，当前的商业冷冻荧光显微镜仅限于低分辨率。为了填补这一空白，我们开发了 CryoSIM，这是一种用于 3D 超分辨率荧光冷冻成像的显微镜，可与冷冻电子显微镜或冷冻软 X 射线断层扫描相关。我们提供了复制仪器的完整说明，主要使用现成的组件和可访问、用户友好的开源 Python 控制软件。因此，cryoSIM 使利用冷冻保存样本的超分辨率荧光成像检测分子的能力变得大众化，以与其细胞超微结构相关。