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Lyophilized Cell-Free Systems Display Tolerance to Organic Solvent Exposure.
ACS Synthetic Biology ( IF 4.7 ) Pub Date : 2020-07-09 , DOI: 10.1021/acssynbio.0c00267
Marilyn S Lee 1 , Rebecca M Raig 2, 3 , Maneesh K Gupta 2 , Matthew W Lux 1
Affiliation  

Cell-free systems offer a powerful way to deliver biochemical activity to the field without cold chain storage. These systems are capable of sensing as well as biosynthesis of useful molecules at the point of need. So far, cell-free protein synthesis (CFPS) reactions have been studied as aqueous solutions in test tubes or absorbed into paper or cloth. Embedding biological functionality into broadly used materials, such as plastic polymers, represents an attractive goal. Unfortunately, this goal has for the most part remained out of reach, presumably due to the fragility of biological systems outside of aqueous environments. Here, we describe a surprising and useful feature of lyophilized cell-free lysate systems: tolerance to a variety of organic solvents. Screens of individual CFPS reagents and different CFPS methods reveal that solvent tolerance varies by CFPS reagent composition. Tolerance to suspension in organic solvents may facilitate the use of polymers to deliver dry cell-free reactions in the form of coatings or fibers, or allow dosing of analytes or substrates dissolved in nonaqueous solvents, among other processing possibilities.

中文翻译:

冻干的无细胞系统显示出对有机溶剂暴露的耐受性。

无细胞系统提供了无需冷链存储即可将生化活性传递到田间的强大方法。这些系统能够在需要时感应和生物合成有用的分子。到目前为止,已经研究了无细胞蛋白质合成(CFPS)反应作为试管中的水溶液或吸收到纸或布中的过程。将生物学功能嵌入广泛使用的材料(例如塑料聚合物)中是一个有吸引力的目标。不幸的是,这个目标在大多数情况下仍然遥不可及,大概是由于水性环境之外的生物系统的脆弱性。在这里,我们描述了冻干的无细胞裂解液系统的令人惊讶和有用的功能:对多种有机溶剂的耐受性。单个CFPS试剂和不同CFPS方法的屏幕显示,溶剂耐受性随CFPS试剂组成而变化。耐受在有机溶剂中的悬浮可以促进聚合物以涂层或纤维的形式传递无细胞反应的干式使用,或允许定量溶解在非水溶剂中的分析物或底物,以及其他处理方法。
更新日期:2020-08-21
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