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Analysis of GPI-anchored proteins involved in germline stem cell proliferation in the Caenorhabditis elegans germline stem cell niche.
The Journal of Biochemistry ( IF 2.7 ) Pub Date : 2020-07-10 , DOI: 10.1093/jb/mvaa075 Marika Rikitake 1 , Ayako Matsuda 1 , Daisuke Murata 1, 2, 3 , Katsufumi Dejima 2, 4 , Kazuko H Nomura 2, 5 , Karen L Abbott 6, 7 , Shohei Mitani 2, 4 , Kazuya Nomura 2, 5, 8
中文翻译:
分析秀丽隐杆线虫生殖细胞干细胞生态位中与生殖细胞干细胞增殖有关的GPI锚定蛋白质。
更新日期:2020-07-10
The Journal of Biochemistry ( IF 2.7 ) Pub Date : 2020-07-10 , DOI: 10.1093/jb/mvaa075 Marika Rikitake 1 , Ayako Matsuda 1 , Daisuke Murata 1, 2, 3 , Katsufumi Dejima 2, 4 , Kazuko H Nomura 2, 5 , Karen L Abbott 6, 7 , Shohei Mitani 2, 4 , Kazuya Nomura 2, 5, 8
Affiliation
Abstract
Stem cells divide and undergo self-renewal depending on the signals received from the stem cell niche. This phenomenon is indispensable to maintain tissues and organs in individuals. However, not all the molecular factors and mechanisms of self-renewal are known. In our previous study, we reported that glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) synthesized in the distal tip cells (DTCs; the stem cell niche) are essential for germline stem cell proliferation in Caenorhabditis elegans. Here, we characterized the GPI-APs required for proliferation. We selected and verified the candidate GPI-APs synthesized in DTCs by RNA interference screening and found that F57F4.3 (GFI-1), F57F4.4 and F54E2.1 are necessary for germline proliferation. These proteins are likely involved in the same pathway for proliferation and activated by the transcription factor PQM-1. We further provided evidence suggesting that these GPI-APs act through fatty acid remodelling of the GPI anchor, which is essential for association with lipid rafts. These findings demonstrated that GPI-APs, particularly F57F4.3/4 and F54E2.1, synthesized in the germline stem cell niche are located in lipid rafts and involved in promoting germline stem cell proliferation in C. elegans. The findings may thus shed light on the mechanisms by which GPI-APs regulate stem cell self-renewal.
中文翻译:
分析秀丽隐杆线虫生殖细胞干细胞生态位中与生殖细胞干细胞增殖有关的GPI锚定蛋白质。
摘要
干细胞分裂并自我更新,具体取决于从干细胞生态位接收的信号。这种现象对于维持个体的组织和器官是必不可少的。但是,并不是所有的分子因素和自我更新的机制是已知的。在我们以前的研究中,我们报道了在末端细小细胞(DTC;干细胞小生境)中合成的糖基磷脂酰肌醇(GPI)-锚定蛋白(GPI-APs)对于秀丽隐杆线虫的生殖系干细胞增殖至关重要。在这里,我们表征了增殖所需的GPI-AP。我们通过RNA干扰筛选选择并验证了在DTC中合成的候选GPI-AP,发现F57F4.3(GFI-1),F57F4.4和F54E2.1是种系增殖所必需的。这些蛋白质可能参与相同的增殖途径,并被转录因子PQM-1激活。我们进一步提供的证据表明,这些GPI-AP通过GPI锚的脂肪酸重塑起作用,这对于与脂筏相关联至关重要。这些发现表明,在种系干细胞生态位中合成的GPI-AP,尤其是F57F4.3 / 4和F54E2.1位于脂筏中,并参与促进秀丽隐杆线虫的种系干细胞增殖。。因此,该发现可能有助于阐明GPI-AP调节干细胞自我更新的机制。