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Circulating and tissue biomarkers as predictors of bromine gas inhalation
Annals of the New York Academy of Sciences ( IF 5.2 ) Pub Date : 2020-07-09 , DOI: 10.1111/nyas.14422
Juan Xavier Masjoan Juncos 1 , Shazia Shakil 1 , Aamir Ahmad 1 , Duha Aishah 1 , Charity J Morgan 2 , Louis J Dell'Italia 3, 4 , David A Ford 5 , Aftab Ahmad 1 , Shama Ahmad 1
Affiliation  

The threat from deliberate or accidental exposure to halogen gases is increasing, as is their industrial applications and use as chemical warfare agents. Biomarkers that can identify halogen exposure, diagnose victims of exposure or predict injury severity, and enable appropriate treatment are lacking. We conducted these studies to determine and validate biomarkers of bromine (Br2) toxicity and correlate the symptoms and the extent of cardiopulmonary injuries. Unanesthetized rats were exposed to Br2 and monitored noninvasively for clinical scores and pulse oximetry. Animals were euthanized and grouped at various time intervals to assess brominated fatty acid (BFA) content in the plasma, lung, and heart using mass spectrometry. Bronchoalveolar lavage fluid (BALF) protein content was used to assess pulmonary injury. Cardiac troponin I (cTnI) was assessed in the plasma to evaluate cardiac injury. The blood, lung, and cardiac tissue BFA content significantly correlated with the clinical scores, tissue oxygenation, heart rate, and cardiopulmonary injury parameters. Total (free + esterified) bromostearic acid levels correlated with lung injury, as indicated by BALF protein content, and free bromostearic acid levels correlated with plasma cTnI levels. Thus, BFAs and cardiac injury biomarkers can identify Br2 exposure and predict the severity of organ damage.

中文翻译:

循环和组织生物标志物作为溴气体吸入的预测因子

故意或意外暴露于卤素气体的威胁正在增加,它们的工业应用和作为化学战剂的用途也在增加。缺乏能够识别卤素暴露、诊断暴露受害者或预测伤害严重程度以及能够进行适当治疗的生物标志物。我们进行了这些研究,以确定和验证溴 (Br2) 毒性的生物标志物,并将症状和心肺损伤程度相关联。未麻醉的大鼠暴露于 Br2 并无创监测临床评分和脉搏血氧饱和度。将动物安乐死并以不同的时间间隔分组,以使用质谱法评估血浆、肺和心脏中的溴化脂肪酸 (BFA) 含量。支气管肺泡灌洗液 (BALF) 蛋白含量用于评估肺损伤。评估血浆中的心肌肌钙蛋白 I (cTnI) 以评估心脏损伤。血液、肺和心脏组织 BFA 含量与临床评分、组织氧合、心率和心肺损伤参数显着相关。总(游离 + 酯化)溴硬脂酸水平与肺损伤相关,如 BALF 蛋白含量所示,游离溴硬脂酸水平与血浆 cTnI 水平相关。因此,BFA 和心脏损伤生物标志物可以识别 Br2 暴露并预测器官损伤的严重程度。如 BALF 蛋白含量所示,游离溴硬脂酸水平与血浆 cTnI 水平相关。因此,BFA 和心脏损伤生物标志物可以识别 Br2 暴露并预测器官损伤的严重程度。如 BALF 蛋白含量所示,游离溴硬脂酸水平与血浆 cTnI 水平相关。因此,BFA 和心脏损伤生物标志物可以识别 Br2 暴露并预测器官损伤的严重程度。
更新日期:2020-07-09
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