Immunohistochemical method to detect avian lymphocytes is an efficient and reliable tool for accurate diagnosis, and immunological analysis of avian diseases. However, there are scarce studies reporting immunohistochemistry (IHC) using commercially available antibodies in formalin-fixed paraffin-embedded (FFPE) chicken tissues. In the present study, we established an immunohistochemical method to identify chicken T and B lymphocytes in FFPE chicken tissues using commercial antibodies against chicken or human antigens. For this IHC method, the five tested anti-T lymphocyte antibodies reacted with chicken T lymphocytes on the FFPE sections. Further, 10 commercial anti-B lymphocyte antibodies were tested; of these, three successfully detected chicken B lymphocytes for IHC. In particular, anti-human CD3 (clone F7.2.38) antibody was most suitable for the detection of chicken T lymphocytes, whereas anti-chicken B cell activating factor receptor (BAFF-R) antibody (clone 2C4) was most suitable for the detection of chicken B lymphocytes under our IHC staining conditions. These two antibodies reacted with numerous lymphocytes of all representative lymphoid tissues without problematic background staining and nonspecific reactions. Our results indicate that T and B lymphocytes in FFPE chicken tissues can be immunohistochemically detected using commercial antibodies.

免疫组织化学方法检测禽淋巴细胞是准确诊断禽类疾病和进行免疫分析的有效而可靠的工具。然而，很少有研究报道在福尔马林固定石蜡包埋（FFPE）鸡组织中使用市售抗体进行免疫组织化学（IHC）。在本研究中，我们建立了一种免疫组织化学方法，使用针对鸡或人抗原的商业抗体来鉴定FFPE鸡组织中的鸡T和B淋巴细胞。对于这种IHC方法，五种测试的抗T淋巴细胞抗体与FFPE切片上的鸡T淋巴细胞反应。此外，测试了10种市售抗B淋巴细胞抗体；其中，三个成功检测出IHC的鸡B淋巴细胞。尤其是抗人类CD3（克隆F7.2。38）抗体最适合检测鸡T淋巴细胞，而抗鸡B细胞活化因子受体（BAFF-R）抗体（克隆2C4）最适合在我们IHC染色条件下检测鸡B淋巴细胞。这两种抗体可与所有代表性淋巴组织的大量淋巴细胞反应，而不会出现有问题的背景染色和非特异性反应。我们的结果表明，FFPE鸡组织中的T和B淋巴细胞可以使用商业抗体进行免疫组织化学检测。这两种抗体可与所有代表性淋巴组织的大量淋巴细胞反应，而不会出现有问题的背景染色和非特异性反应。我们的结果表明，FFPE鸡组织中的T和B淋巴细胞可以使用商业抗体进行免疫组织化学检测。这两种抗体可与所有代表性淋巴组织的大量淋巴细胞反应，而不会出现有问题的背景染色和非特异性反应。我们的结果表明，FFPE鸡组织中的T和B淋巴细胞可以使用商业抗体进行免疫组织化学检测。