The influence of exposing carcinoma cells to a static magnetic field (SMF) and low-intensity pulsed ultrasound (LIPUS), for different durations (15–45 min/d), in the presence of magnetic and non-magnetic drug carriers, on their in vitro inhibition is examined. Increasing the exposure time by 15 min/d decreased the culture duration by 24 h to achieve the same level of inhibition in colon (HCT116) and hepatocellular (HepG2) cells. Cell cycle analysis revealed enhanced cellular blockage in G1 and S phases with SMF + LIPUS exposure, and exposure for 45 min/d completely suppressed the S → G2 transition. Apoptosis of both types of cells increased with SMF + LIPUS treatment time, and HepG2 cells exhibited elevated necrosis with >30 min/d exposure. HepG2 cells also had higher amounts of reactive oxygen species (seven- to eightfold) than HCT116 cells (two- to sixfold), suggesting treatment effectiveness is cell and drug carrier dependent. The accelerated cellular activities are attributed to the enhanced internalization of drug carriers as a consequence of destabilized cellular membranes caused by the SMF + LIPUS-generated mechanical and electrical stimuli.

在磁性和非磁性药物载体存在的情况下，将癌细胞暴露于静磁场 (SMF) 和低强度脉冲超声 (LIPUS) 不同持续时间（15-45 分钟/天）对其的影响体外检查抑制。将暴露时间增加 15 分钟/天，培养持续时间减少 24 小时，以在结肠 (HCT116) 和肝细胞 (HepG2) 细胞中达到相同的抑制水平。细胞周期分析显示 SMF + LIPUS 暴露在 G1 和 S 期增强细胞阻塞，暴露 45 分钟/天完全抑制 S→G2 转变。随着 SMF + LIPUS 处理时间的增加，两种类型的细胞的细胞凋亡都增加，并且 HepG2 细胞在暴露 >30 分钟/天时表现出更高的坏死。HepG2 细胞还具有比 HCT116 细胞（2 到 6 倍）更多的活性氧（7 到 8 倍），这表明治疗效果取决于细胞和药物载体。