Autologous fibrin has been widely used in surgical procedures for both soft and hard tissue repair. There are different protocols and devices to obtain this matrix, with varying centrifugal time, gravity force, speed, angle of the sample tube and spinning radius. The aim of this study was to compare three methods of obtaining autologous fibrin: L-PRF using the Intra-Spin L-PRF centrifuge (Dohan protocol), the advanced PRF (A-PRF) using the Intra-Spin L-PRF centrifuge and autologous leukocyte fibrin (ALF), using the Kasvi centrifuge. Venous blood was collected from 7 healthy volunteers, which were submitted to the 3 different methods of centrifugation. The membranes were tissue-processed and evaluated by immunohistochemistry for CD3, CD20, CD68 and CD138. For CD68+, a lower number of cells was immunolabelled in the L-PRF group when compared to the other groups (A-PRF and ALF). For CD3+, a lower number of immunolabellated cells was observed in the ALF group when compared to the remaining groups (p < 0.05). In the A-PRF group, the CD20+ cell count was lower than in the remaining groups. No difference was observed in CD138+ cell counts between the groups. The 3 protocols tested are suitable for obtaining autologous fibrin membranes.

自体纤维蛋白已广泛用于软组织和硬组织修复的外科手术。有不同的协议和设备来获得这个矩阵，具有不同的离心时间、重力、速度、样品管的角度和旋转半径。本研究的目的是比较获得自体纤维蛋白的三种方法：使用 Intra-Spin L-PRF 离心机（Dohan 协议）的 L-PRF、使用 Intra-Spin L-PRF 离心机的高级 PRF (A-PRF) 和自体白细胞纤维蛋白 (ALF)，使用 Kasvi 离心机。从 7 名健康志愿者收集静脉血，将其提交给 3 种不同的离心方法。对膜进行组织处理并通过免疫组织化学对 CD3、CD20、CD68 和 CD138 进行评估。对于 CD68+，与其他组（A-PRF 和 ALF）相比，L-PRF 组中免疫标记的细胞数量较少。对于 CD3+，与其余组相比，ALF 组中观察到的免疫标记细胞数量较少（p < 0.05）。在 A-PRF 组中，CD20+ 细胞计数低于其余组。组间 CD138+ 细胞计数没有观察到差异。测试的 3 个协议适用于获得自体纤维蛋白膜。