Abstract The present study was carried out to identify single nucleotide variations in inhibin alpha (INHA)geneto evaluate its association with production traits in Malabari goats. A total of 251 Malabari adult goats were screened for polymorphism using PCR-SSCP.Representative samples of each SSCP pattern were sequenced to ascertain the genotypes. Five fragments, 274 bp, 220 bp, 160 bp, 287 bp and 253 bp covering the two exons of INHA were analyzed. The 274 bp fragment showed two diplotypes (AA and AB) and sequencing revealed one synonymous SNP (c.129 G > A) in exon 1. The AA genotype showed significant (p ≤ 0.05) association with body length. Similar monomorphic band pattern was observed for 220 bp fragment of exon 2. The 160 bp fragment exhibited two genotypes DD and DE with a synonymous SNP c.567 G > A in exon 2. Goats carrying DD genotype had significantly (p ≤ 0.05) higher adult body weight and body length compared to DE genotype. Three diplotypes, HH, HJ and JJ with a synonymous SNP, c.651 G > A were observed at 287 bp fragment. Significantly (p C. Genotypes of INHA 253 bp (exon 2) locus had significant (p ≤ 0.05) influence on litter size, body weight and trunk circumference. The PP and PQ showed a significantly higher litter size and trunk circumference (p ≤ 0.05) compared to QQ genotypes.Present study indicated the potential role of INHA gene on reproduction and growth, hence it can be used as a candidate gene in the improvement of Malabari goats.

中文翻译：

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抑制素α基因多态性与印度马拉巴里山羊产仔数和生长的关联

摘要 本研究旨在鉴定抑制素α (INHA) 基因中的单核苷酸变异，以评估其与马拉巴里山羊生产性状的关联。使用 PCR-SSCP 对总共 251 只马拉巴里成年山羊进行了多态性筛选。对每个 SSCP 模式的代表性样本进行测序以确定基因型。分析了覆盖 INHA 两个外显子的五个片段，274 bp、220 bp、160 bp、287 bp 和 253 bp。274 bp 片段显示出两种双倍型（AA 和 AB），测序显示外显子 1 中有一个同义 SNP（c.129 G > A）。AA 基因型显示与体长显着（p ≤ 0.05）相关。对于外显子 2 的 220 bp 片段观察到类似的单态带模式。 160 bp 片段表现出两种基因型 DD 和 DE，在外显子 2 中具有同义 SNP c.567 G > A。与 DE 基因型相比，携带 DD 基因型的山羊的成年体重和体长显着（p ≤ 0.05）更高。在 287 bp 片段处观察到三个双倍型，HH、HJ 和 JJ，具有同义 SNP，c.651 G > A。显着（p C. INHA 253 bp（外显子 2）基因座的基因型对窝产仔数、体重和躯干围有显着影响（p ≤ 0.05）。PP 和 PQ 显示出明显更高的窝产仔数和躯干围（p ≤ 0.05 ) 与QQ 基因型相比。目前的研究表明INHA 基因在繁殖和生长方面具有潜在作用，因此可以作为候选基因用于马拉巴里山羊的改良。