Eye motion is a major confound for magnetic resonance imaging (MRI) in neuroscience or ophthalmology. Currently, solutions toward eye stabilisation include participants fixating or administration of paralytics/anaesthetics. We developed a novel MRI protocol for acquiring 3-dimensional images while the eye freely moves. Eye motion serves as the basis for image reconstruction, rather than an impediment. We fully reconstruct videos of the moving eye and head. We quantitatively validate data quality with millimetre resolution in two ways for individual participants. First, eye position based on reconstructed images correlated with simultaneous eye-tracking. Second, the reconstructed images preserve anatomical properties; the eye’s axial length measured from MRI images matched that obtained with ocular biometry. The technique operates on a standard clinical setup, without necessitating specialized hardware, facilitating wide deployment. In clinical practice, we anticipate that this may help reduce burdens on both patients and infrastructure, by integrating multiple varieties of assessments into a single comprehensive session. More generally, our protocol is a harbinger for removing the necessity of fixation, thereby opening new opportunities for ethologically-valid, naturalistic paradigms, the inclusion of populations typically unable to stably fixate, and increased translational research such as in awake animals whose eye movements constitute an accessible behavioural readout.

眼动是神经科学或眼科磁共振成像 (MRI) 的主要混淆因素。目前，稳定眼睛的解决方案包括参与者固定或使用麻痹剂/麻醉剂。我们开发了一种新颖的 MRI 协议，用于在眼睛自由移动时获取 3 维图像。眼动是图像重建的基础，而不是障碍。我们完全重建了移动的眼睛和头部的视频。我们以两种方式为个体参与者以毫米分辨率定量验证数据质量。首先，基于与同步眼动追踪相关的重建图像的眼部位置。其次，重建的图像保留了解剖学特性；从 MRI 图像测量的眼睛轴向长度与眼部生物测量获得的相匹配。该技术在标准临床设置上运行，不需要专门的硬件，便于广泛部署。在临床实践中，我们预计通过将多种评估整合到一个综合会议中，这可能有助于减轻患者和基础设施的负担。更一般地说，我们的协议是消除注视必要性的先兆，从而为行为学上有效的自然范式开辟了新的机会，包括通常无法稳定注视的人群，以及增加的转化研究，例如眼睛运动构成的清醒动物一个可访问的行为读数。我们预计，通过将多种评估整合到一个综合会议中，这可能有助于减轻患者和基础设施的负担。更一般地说，我们的协议是消除注视必要性的先兆，从而为行为学上有效的自然范式开辟了新的机会，包括通常无法稳定注视的人群，以及增加的转化研究，例如眼睛运动构成的清醒动物一个可访问的行为读数。我们预计，通过将多种评估整合到一个综合会议中，这可能有助于减轻患者和基础设施的负担。更一般地说，我们的协议是消除注视必要性的先兆，从而为行为学上有效的自然范式开辟了新的机会，包括通常无法稳定注视的人群，以及增加的转化研究，例如眼睛运动构成的清醒动物一个可访问的行为读数。