Caseous lymphadenitis is a chronic disease of goats caused by Corynebacterium pseudotuberculosis (C.pseudotuberculosis) which causes great harm to the dairy goats industry. In order to obtain detailed information about the pathogenesis and host immune response in C.pseudotuberculosis-infected goats, in this study, the gene expression difference of spleen tissue after infection with C.pseudotuberculosis was analyzed by high-throughput sequencing. Transcripts obtained over 412 700 462 clean reads after reassembly were 21 343 genes detected, of which 14 720 were known genes and 7623 new genes were predicted. There were 448 up-regulated and 519 down-regulated differentially expressed genes (DEGs). Gene Ontology (GO) analysis indicated that all of the DEGs were annotated into biological process, cellular component and molecular function. Most of these unigenes are annotated in cellular processes, the cell and binding. KEGG analysis of the DEGs showed that a total of 8733 DEGs unigenes were annotated into 459 pathways classified into 6 main categories. Most of these annotated unigenes were related to immune system response to the infectious diseases pathways. In addition, 14 DEGs were verified by quantitative real-time PCR. As the first, in vivo, RNAseq analysis of dairy goats and C.pseudotuberculosis infection, this study provides knowledge about the transcriptomics of spleen in C.pseudotuberculosis-infected goats, from which a complex molecular pathways and immune response mechanism are involved in C.pseudotuberculosis infection.

干酪性淋巴结炎是由假结核棒杆菌（C.pseudotuberculosis）引起的山羊的慢性疾病，对乳山羊业造成极大的伤害。为了获得有关感染假结核杆菌的山羊的发病机理和宿主免疫反应的详细信息，在本研究中，我们研究了感染假结核杆菌后脾脏组织的基因表达差异。通过高通量测序进行分析。重组后通过412 700 462次完整读取获得的转录本为21 343个基因，其中14 720个为已知基因，并且预测了7623个新基因。存在448个上调和519个下调的差异表达基因（DEG）。基因本体论（GO）分析表明，所有DEG均被注释为生物过程，细胞成分和分子功能。这些单基因中的大多数在细胞过程，细胞和结合中都有注释。KEGG对DEG的分析表明，共有8733个DEGs单基因被注释为459条途径，分为6个主要类别。这些带注释的单基因大多数与免疫系统对传染病途径的反应有关。另外，通过定量实时PCR验证了14个DEG。首先，在体内，C.pseudotuberculosis感染，这项研究提供了有关在C.pseudotuberculosis感染的山羊中脾转录组学的知识，其中C.pseudotuberculosis感染涉及复杂的分子途径和免疫应答机制。