To increase the carbon fixation efficiency of Nannochloropsis oceanica cultivated with industrial flue gas and wastewater, typical CO₂ suppliers of methanol, NaHCO₃ and CO₂ were combined with 15 % CO₂ flue gas, then compared for their effects on N. oceanica intracellular metabolic and biological regulation. In the presence of CO₂ gas with and without its ionized acid (HCO₃⁻), microalgal growth was inhibited due to carbon transport-dependent ion transfer and balance. In a weakly acidic environment, growth inhibition was due to cell apoptosis, while in a weakly alkaline environment this was led to cell division inhibition by a decrease of translation initiation factors and carbon fixation stagnation by mass closure of photosynthetic reaction centers. When methanol was combined with 15 % CO₂ gas, less negative effects on N. oceanica growth due to changes in the CO₂/HCO₃- equilibrium. The presence of methanol increased light-dependent reactions by providing sufficient substrates for photosynthetic pigment synthesis through enhanced glycolysis. Total carbon fixation was further increased by higher rates of cell division. The biomass yield and carbon fixation efficiency of N. oceanica grown with methanol and 15 % CO₂ was 8.4-fold, and 78.3 % greater, respectively, than that of N. oceanica grown with only 15 % CO₂ gas.

为了提高用工业烟气和废水培养的拟南芥的固碳效率，将典型的甲醇，NaHCO ₃和CO _2的CO ₂供应商与15％的CO ₂烟气结合，然后比较它们对大肠念珠菌细胞内代谢的影响。和生物调节。在CO存在₂气体有和没有其离子化的酸（HCO ₃ ^-），由于依赖于碳传输的离子转移和平衡，微藻的生长受到抑制。在弱酸性环境中，生长抑制是由于细胞凋亡引起的，而在弱碱性环境中，这是由于翻译起始因子的减少和光合反应中心质量封闭引起的碳固定停滞而导致的细胞分裂抑制。当甲醇与15％CO ₂气体混合使用时，由于CO ₂ / HCO _3的变化而对大洋猪笼草生长的负面影响较小-平衡。甲醇的存在通过提供增强糖酵解的光合色素合成所需的底物，增加了光依赖性反应。更高的细胞分裂速率进一步提高了总碳固定率。用甲醇和15％CO ₂种植的大叶猪笼草的生物量产量和固碳效率分别比仅用15％CO ₂气体种植的大叶猪笼草的生物量产量和固碳效率分别高8.4倍和78​​.3％。