In this study, a simple, sensitive and efficient analytical method was developed and validated for the determination of 11 fungicides in grape, potato, rice, corn, wheat and soybean using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS). The fungicides were extracted using acetonitrile, and then the extracts were cleaned up using primary secondary amine (PSA), octadecylsilane (C18) and graphitized carbon black (GCB). Method validation displayed acceptable linearities for all pesticides (R² greater than 0.99). The calculated limits of detection and quantification were 1 μg kg⁻¹ and 10 μg kg⁻¹ in different matrices, respectively. It was necessary to employ matrix-matched standards for correct quantification of these fungicides in all matrices. Acceptable recoveries (75.7–104.2%) were obtained for all the target compounds with good intra-day and inter-day precisions (RSD ≤ 11.9%). The results indicated that the method is reliable for detecting these fungicides in various foods.

在这项研究中，开发了一种简单，灵敏且高效的分析方法，并通过使用高效液相色谱-串联质谱（UHPLC–MS / MS）测定葡萄，马铃薯，大米，玉米，小麦和大豆中的11种杀菌剂进行了验证。多发性硬化症）。使用乙腈提取杀真菌剂，然后使用伯仲胺（PSA），十八烷基硅烷（C18）和石墨化炭黑（GCB）净化提取物。方法验证显示所有农药的线性均可接受（R ² 大于0.99）。计算的检测和定量限为1μgkg ^-1和10μgkg ^-1分别在不同的矩阵中 必须使用基质匹配的标准品来正确定量所有基质中的这些杀菌剂。所有目标化合物的日内和日间精密度（RSD≤11.9％）都达到了可接受的回收率（75.7–104.2％）。结果表明该方法可用于多种食品中这些杀菌剂的检测。