Bacteria from the genus Halomonas hold promise in biotechnology as sources of salt-tolerant enzymes, biosurfactants, biopolymers, osmolytes, and as actors in bioremediation processes. In a previous work, we have identified Halomonas socia strain CKY01 having various hydrolase activities. Here, we aimed to study the survival strategies of marine bacteria. A deep genome sequencing study of H. socia CKY01 has revealed 4627 genes reaching 4,753,299 bp with 64 % of GC content. This strain produced polyhydroxybutyrate (PHB) having one gene clusters having phaC and phasin, and it has several genes responsible for the uptake, synthesis, and transport of the osmolytes such as betaine, choline, ectoine, carnitine, and proline in the bacterial genome. The addition of 60 mM glutamate, 60 mM proline and 60 mM ectoine enhanced growth 300.8 %, 294.2 % and 235.0 %, respectively, under 10 % saline conditions. In particular, ectoine and proline increased salt resistance and allowed cells to survive in more than 15 % NaCl. By combining experimental and genome sequencing data, we have investigated the importance of osmolytes on the survival of this Halomonas strain.

盐单胞菌属的细菌在生物技术中有望作为耐盐酶，生物表面活性剂，生物聚合物，渗透液的来源，并在生物修复过程中发挥作用。在以前的工作中，我们确定了具有各种水解酶活性的嗜盐单胞菌菌株CKY01。在这里，我们旨在研究海洋细菌的生存策略。幽门螺杆菌的深层基因组测序研究CKY01已揭示4627个基因，达到4,753,299 bp，GC含量为64％。该菌株产生了具有一个具有phaC和phasin的基因簇的聚羟基丁酸酯（PHB），并且它具有几个在细菌基因组中负责渗透液的吸收，合成和运输的基因，如甜菜碱，胆碱，ectoine，肉碱和脯氨酸。在10％的生理盐水条件下，添加60 mM谷氨酸，60 mM脯氨酸和60 mM植酸分别分别促进了300.8％，294.2％和235.0％的生长。特别是，油菜碱和脯氨酸提高了耐盐性，并使细胞在超过15％的NaCl中存活。通过结合实验和基因组测序数据，我们研究了渗透压对这种Halomonas菌株存活的重要性。