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Three phase partitioning for simultaneous extraction of oil, protein and polysaccharide from rice bran
Innovative Food Science & Emerging Technologies ( IF 6.6 ) Pub Date : 2020-07-10 , DOI: 10.1016/j.ifset.2020.102447
Hong Wang , Haoyuan Geng , Jun Chen , Xu Wang , Dan Li , Tong Wang , Dianyu Yu , Liqi Wang

Three phase partitioning (TPP) technique was used to extract oil, protein and polysaccharides simultaneously from rice bran. The fatty acid composition of rice bran oil (RBO) and structure of rice bran protein (RBP) and rice bran polysaccharides (RBPS) were analyzed. Under the optimal conditions of (NH4)2SO4 concentration 28% (w/v), slurry to t-butanol ratio 1:1.1 (v/v), pH 5.10, extraction temperature 40 °C and extraction time 1 h, the highest extraction yields of RBO, RBP and RBPS were 17.28%, 6.81% and 2.09%, respectively. The fatty acid composition of RBO was analyzed by GC, the structure of RBP was analyzed by FTIR and SDS-PAGE, and the structure of RBPS was analyzed by FTIR. The results were similar to those obtained by other methods. Therefore, TPP can be used as an effective technology for simultaneous extraction of oil, protein and polysaccharide from rice bran.



中文翻译:

三相分配同时从米糠中提取油,蛋白质和多糖

三相分配(TPP)技术用于从米糠中同时提取油,蛋白质和多糖。分析了米糠油(RBO)的脂肪酸组成,米糠蛋白(RBP)和米糠多糖(RBPS)的结构。在(NH 42 SO 4的最佳条件下浓度28%(w / v),浆液与叔丁醇之比1:1:1(v / v),pH 5.10,提取温度40°C,提取时间1 h,RBO,RBP和RBPS的最高提取产率为17.28 %,6.81%和2.09%。用GC分析RBO的脂肪酸组成,用FTIR和SDS-PAGE分析RBP的结构,用FTIR分析RBPS的结构。结果类似于通过其他方法获得的结果。因此,TPP可以用作从米糠中同时提取油,蛋白质和多糖的有效技术。

更新日期:2020-07-16
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