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Methylglyoxal-hydroimidazolones (MG-Hs) instead of Nɛ-(carboxymethyl)-l-lysine (CML) is the major advanced glycation end-product during drying process in black tea.
Food Chemistry ( IF 8.8 ) Pub Date : 2020-07-10 , DOI: 10.1016/j.foodchem.2020.127499
Wei Zhang 1 , Beibei Zhang 2 , Yang Ye 3 , Hongkai Zhu 4
Affiliation  

This study was to examine the formation of advanced glycation end-products (AGEs) in black tea during drying process at 90, 120, and 150 °C for 1 h. Nine AGEs including Nɛ-(carboxyethyl)-l-lysine (CEL), Nɛ-(carboxymethyl)-l-lysine (CML), three isomers of methylglyoxal-hydroimidazolones (MG-Hs), three isomers of glyoxal-hydroimidazolones (GO-Hs), and argpyrimidine were quantified by using HPLC-MS/MS with isotope-labelled internal standard. Results showed that each AGE during the drying process of 150 °C was significantly higher (p < 0.05) than at 90 and 120 °C, and argpyrimidine was only found in the treatment of 150 °C. MG-H1/3 was first quantified as the major AGE during drying at 120–150 °C, the content respectively reached to (39.66 ± 2.61) μg/g and (58.88 ± 1.76) μg/g after 1 h drying, where CML content only had (19.86 ± 1.02) μg/g and (23.71 ± 1.40) μg/g. This study indicated that arginine derived-AGEs are the key components of black tea AGEs.



中文翻译:

甲基乙二醛-氢咪唑啉酮(MG-Hs)代替N 1-(羧甲基)-1-赖氨酸(CML)是红茶干燥过程中主要的高级糖化终产物。

这项研究旨在检查在90、120和150°C干燥1小时的过程中红茶中高级糖基化终产物(AGEs)的形成。九点的AGEs包括Nɛ-(羧乙基) --赖氨酸(CEL),Nɛ-(羧甲基) --赖氨酸(CML),甲基乙二醛-氢咪唑啉酮(MG-Hs)的三个异构体,乙二醛-氢咪唑啉酮(GO-Hs)和argpyrimidine的三个异构体通过具有同位素标记内标的HPLC-MS / MS进行定量。结果表明,在150°C的干燥过程中,每个AGE均显着高于90和120°C(p <0.05),仅在150°C的处理中才发现精氨嘧啶。MG-H1 / 3首先被量化为在120–150°C下干燥过程中的主要AGE,干燥1 h后其含量分别达到(39.66±2.61)μg/ g和(58.88±1.76)μg/ g,其中CML含量仅为(19.86±1.02)μg/ g和(23.71±1.40)μg/ g。这项研究表明,精氨酸衍生的AGEs是红茶AGEs的关键成分。

更新日期:2020-07-14
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