Growth differentiation factor 9 (GDF9) plays pivotal roles in regulating follicular development in many mammalian species. In the present study, the full-length Schizothorax prenanti gdf9 cDNA sequence was isolated and characterized, and its expression pattern in developing gonads and in the gonads of exogenous oestradiol (E₂)-fed fish were analysed. The S. prenanti gdf9 cDNA sequence consisted of 1958 base pairs (bp), encoded a 413 amino acid, and showed high sequence similarity with Carassius gibelio and Cyprinus carpio. The quantitative real-time PCR analysis revealed that gdf9 was mainly expressed in the gonads, with particularly high expression in the cortical alveoli stage ovary and late-spermatogenic stage testis. Immunohistochemical signals for Gdf9 were mainly detected in chromatin nucleolar oocytes, spermatogonia and spermatocytes. Furthermore, the gonadal expression of gdf9 induced by exogenous E₂ was related to the feeding time and dose. Taken together, these findings were helpful to gain a better understanding of the role of Gdf9 in both folliculogenesis and spermatogenesis in S. prenanti.

生长分化因子9（GDF9）在调节许多哺乳动物物种的卵泡发育中起关键作用。在本研究中，分离并鉴定了全长裂殖酵母prenanti gdf9 cDNA序列，并分析了其在发育中的性腺和外源雌二醇（E ₂）喂养的鱼的性腺中的表达模式。的S. prenanti GDF9 cDNA序列由1958个碱基对（bp）的，编码的413个氨基酸，并表现出较高的序列相似性以鲫鱼鲫和鲤鱼。实时定量PCR分析表明gdf9在性腺中主要表达，在皮质肺泡期卵巢和晚期生精期睾丸中特别高表达。Gdf9的免疫组织化学信号主要在染色质核仁卵母细胞，精原细胞和精母细胞中检测到。此外，外源E ₂诱导的gdf9的性腺表达与饲养时间和剂量有关。综上所述，这些发现有助于更好地理解Gdf9在葡萄球菌卵泡发生和精子发生中的作用。