Phospholipids are building blocks of biological membranes having a key role in cellular functionality. The presence of unsaturated fatty acids in their conformation makes them prompt to oxidation reactions, leading to dysfunctions of living cells or to instability of lipid containing food products. The aim of this review is to gather together the latest advances on the understanding on lipids' peroxidation, using liposomes as model systems, including the main available analytical methods to monitor peroxidation reactions, with special emphasis on Fourier Transform Infrared (FTIR) and Raman spectroscopies. Lipid peroxidation is the most widely studied free radical chain reaction, which occurs in three steps: initiation, propagation and termination, making difficult to determine peroxidation products. Using liposomes as model membrane systems provides a useful tool to investigate the effects of free radicals. Different analytical methods enable the determination of peroxidation primary or secondary products. In particular, FTIR and Raman spectroscopies allow the simultaneous determination of peroxidation products in a non-destructive and easy-to-use manner. A quick monitoring of both reagents and products provides a reliable method for the quality control of industrial products or even for diagnostics, thus underlying the strong potential of vibrational spectroscopic based techniques.

磷脂是生物膜的组成部分，在细胞功能中起着关键作用。不饱和脂肪酸在其构象中的存在促使它们发生氧化反应，导致活细胞功能障碍或含脂食品不稳定。这篇综述的目的是收集关于脂质过氧化的最新进展，使用脂质体作为模型系统，包括监测过氧化反应的主要可用分析方法，特别强调傅里叶变换红外 (FTIR) 和拉曼光谱. 脂质过氧化是研究最广泛的自由基链式反应，它发生在三个步骤：引发、传播和终止，因此难以确定过氧化产物。使用脂质体作为模型膜系统提供了一种有用的工具来研究自由基的影响。不同的分析方法可以测定过氧化初级或次级产物。特别是，FTIR 和拉曼光谱允许以无损且易于使用的方式同时测定过氧化产物。对试剂和产品的快速监测为工业产品的质量控制甚至诊断提供了一种可靠的方法，从而奠定了基于振动光谱技术的巨大潜力。FTIR 和拉曼光谱允许以无损且易于使用的方式同时测定过氧化产物。对试剂和产品的快速监测为工业产品的质量控制甚至诊断提供了一种可靠的方法，从而奠定了基于振动光谱技术的巨大潜力。FTIR 和拉曼光谱允许以无损且易于使用的方式同时测定过氧化产物。对试剂和产品的快速监测为工业产品的质量控制甚至诊断提供了一种可靠的方法，从而奠定了基于振动光谱技术的巨大潜力。