The aquatic ecosystem is the ultimate sink for consumer and industrial waste discharge that contains nanometals such as titanium dioxide nanoparticle (n-TiO₂). In this environment, nutrient availability and nanometals influence phytoplankton community structure and function. In a mesocosm experiment, we evaluated the interactive effect of n-TiO₂ (48 mg L⁻¹) and two nitrogen (N) levels (limited, LN; and replete, HN) on the phytoplankton community structure (biomass, species diversity and richness, algal species divisions), biochemical composition (carbohydrates, proteins, and lipids), and antioxidant response (peroxidase activity, POD). n-TiO₂ decreased total phytoplankton biomass, and its combination with HN led to the highest decrease. Species diversity was not affected by N level, n-TiO₂, and their interaction, while species richness decreased in combined n-TiO₂ and HN treatment_. All these recorded effects of n-TiO₂ on the phytoplankton community structure were enhanced by increasing temperature over time. LN initially reduced phytoplankton carbohydrate content but increased by the presence of n-TiO₂ and its interaction with N levels. Total protein and lipid content were not affected by n-TiO₂ or its interaction with N levels. POD activity was increased by the interaction between n-TiO₂ and the N levels tested. Our results indicate that the influence of n-TiO₂ on the phytoplankton community was dependent on the concentration of N. Also, phytoplankton carbohydrate content and community structure varied with increasing water temperature. A few species thrived concerning biomass during exposure to the LN + n-TiO₂ (Scenedesmus quadricauda, Coelastrum reticulum, and Microcystis sp.) and HN + n-TiO₂ (Microcystis sp.) treatments. Members of the Chlorophyta were generally susceptible to the presence of n-TiO₂ regardless of the N level. Thus, the presence of n-TiO₂ in aquatic ecosystems can alter phytoplankton community structure and dynamics.

水生生态系统是消费和工业废物排放的最终汇，其中包含纳米金属，例如二氧化钛纳米颗粒（n-TiO ₂）。在这种环境下，养分的可利用性和纳米金属会影响浮游植物的群落结构和功能。在中观宇宙实验中，我们评估了n-TiO ₂（48 mg L ^-1）和两个氮（N）水平（有限的LN；以及充足的HN）对浮游植物群落结构（生物量，物种多样性和丰富度，藻类物种划分），生化成分（碳水化合物，蛋白质和脂质）和抗氧化剂响应（过氧化物酶活性，POD）。纳米TiO ₂浮游植物总生物量减少，并且其与HN的组合导致降幅最大。氮，n-TiO ₂及其相互作用对物种多样性没有影响，而n-TiO ₂和HN联合处理可降低物种丰富度_。随着时间的推移，温度升高增强了所有这些记录的n-TiO ₂对浮游植物群落结构的影响。LN最初降低了浮游植物碳水化合物含量，但由于存在n-TiO ₂及其与氮水平的相互作用而增加。总蛋白质和脂质含量不受n-TiO ₂或其与氮水平的相互作用的影响。POD活性通过n-TiO ₂之间的相互作用而增加和N水平测试。我们的结果表明，n-TiO ₂对浮游植物群落的影响取决于氮的浓度。此外，浮游植物碳水化合物含量和群落结构随水温的升高而变化。在暴露于LN + n-TiO ₂（Scendesmus quadricauda，网纹夜蛾和Microcystis sp。）和HN + n-TiO ₂（Microcystis sp。）处理期间，有关生物量的物种激增。不论氮水平如何，绿藻的成员通常容易受到n-TiO ₂的影响。因此，n-TiO ₂的存在 在水生生态系统中可以改变浮游植物的群落结构和动态。