Poly(ADP-ribose) polymerases (PARP) are involved in the regulation of plant growth and development, especially under stress conditions. These enzymes post-translationally modify target proteins by adding ADP-ribose polymers. This study presents the effect of PARP chemical inhibitor 3-methoxybenzamide (3 MB) on in vitro plant growth, microtuber formation, and Agrobacterium-mediated transformation of blue potato (Solanum tuberosum L. subsp. andigenum). The addition of 3 MB (0.2 to 0.6 mM) significantly increased the growth and microtuber formation of in vitro propagated plants. The highest gain in plant fresh weight, root mass, and microtuberization was observed in clonal propagation medium with 0.4 mM 3 MB. Transformation frequency of blue potato internodes increased to 43%, 2.7-fold over the non-treated control (16%), when 0.2 mM 3 MB was included in the culture medium during the whole process. The addition of 3 MB during Agrobacterium transformation did not affect transgene copy number in regenerated plants. Southern blot analyses and histochemical staining for ß-glucuronidase activity confirmed the presence and expression, respectively, of the uidA transgene in plant tissues. From these results, it can be concluded that the inhibition of PARP activity can increase biomass production and transformation rates for genetic improvement of blue potato.

聚（ADP-核糖）聚合酶（PARP）参与植物生长和发育的调节，特别是在胁迫条件下。这些酶通过添加ADP-核糖聚合物来翻译后修饰靶蛋白。这项研究提出了PARP化学抑制剂3-甲氧基苯甲酰胺（3 MB）对蓝薯（Solanum tuberosum L. subsp。andigenum）的体外植物生长，微块茎形成和农杆菌介导的转化的影响。添加3 MB（0.2至0.6 mM）可显着增加体外生长和微块茎形成繁殖的植物。在0.4 mM 3 MB的无性繁殖培养基中观察到最高的植物鲜重，根质量和微块茎化。当在整个过程中将0.2 mM 3 MB包含在培养基中时，蓝马铃薯节间的转化频率增加到43％，是未处理对照（16％）的2.7倍。在农杆菌转化过程中添加3 MB不会影响再生植物中的转基因拷贝数。Southern印迹分析和ß-葡萄糖醛酸苷酶活性的组织化学染色分别证实了uidA的存在和表达。植物组织中的转基因。从这些结果可以得出结论，PARP活性的抑制可以增加生物量的产生和转化率，以改善蓝马铃薯的遗传。