Pikeperch (Sander lucioperca L.) is a freshwater fish species of major economic importance in several producer countries. Insufficient information about growth-related genes in pikeperch prevents their breeding through marker-assisted selection (MAS). This study aimed to clone growth-related genes (IGF-I, IGF-II, and GHR) of pikeperch to detect single-nucleotide polymorphisms (SNPs) associated with growth, laying the foundation for MAS in pikeperch. Full-length cDNA sequences of IGF-I, IGF-II, and GHR were isolated using RACE PCR; the cDNA sequences obtained were 883, 1210, and 1995 base pairs, respectively, encoding proteins of 183, 215, and 664 amino acids. The three genes were expressed in all tissues tested, though at especially high levels in the liver. Four SNPs, comprising two in intron 1 of IGF-I, one in intron 3 of IGF-II, and one in the coding region of GHR, were found in a cultured population of pikeperch. Three of the SNPs were successfully genotyped by Kompetitive allele–specific PCR in a randomly mixed group of 298 individuals. The SNP in IGF-II was significantly associated with body weight (SL2-IGF-II-c.544+1111_544+1112delAAinsTC; allele frequency, AA:TC = 52.8:47.2; AA-AA (26), AA-TC (54), and TC-TC (20) genotypes). The fish with genotype TC-TC of SL2-IGF-II-c.544+1111_544+1112delAAinsTC had a significantly greater body weight than fish of genotype AA-AA (P < 0.05). This discovery may provide a useful marker for marker-assisted selection of pikeperch in the future.

鲈鱼（Sander lucioperca L.）是在几个生产国具有重要经济意义的淡水鱼类。鲈鱼中与生长相关基因的信息不足，无法通过标记辅助选择（MAS）进行繁殖。这项研究旨在克隆与鲈鱼生长相关的基因（IGF-I，IGF-II和GHR），以检测与生长相关的单核苷酸多态性（SNP），从而为鲈鱼中的MAS奠定基础。IGF-I，IGF-II和GHR的全长cDNA序列使用RACE PCR分离；获得的cDNA序列分别为883、1210和1995个碱基对，编码183、215和664个氨基酸。这三个基因在所有测试的组织中都有表达，尽管在肝脏中含量特别高。在鲈鱼的培养种群中发现了四个SNP，包括两个IGF-I内含子1 ，一个IGF-II内含子3和一个GHR编码区。通过竞争性等位基因特异性PCR成功地在298个随机混合组中对其中的三个SNP进行了基因分型。IGF-II中的SNP与体重显着相关（SL2-IGF-II-c.544 + 1111_544 + 1112delAAinsTC;等位基因频率，AA：TC = 52.8：47.2; AA-AA（26），AA-TC（54）和TC-TC （20）基因型）。具有SL2-IGF-II-c.544 + 1111_544 + 1112delAAinsTC基因型TC-TC的鱼的体重明显高于基因型AA-AA的鱼（P  <0.05）。这一发现可能为将来的标记辅助选择鲈提供有用的标记。