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Effect of trace metals on growth performance and accumulation of lipids, proteins, and carbohydrates on the green microalga Scenedesmus obliquus
Aquaculture International ( IF 2.9 ) Pub Date : 2020-03-23 , DOI: 10.1007/s10499-020-00533-0
Carlos Yure Barbosa de Oliveira , Thayna Lie Viegas , Maria Fernanda Oliveira da Silva , Debora Machado Fracalossi , Rafael Garcia Lopes , Roberto Bianchini Derner

Microalgae biomass is considered one of the most promising raw materials for the development of high commercial value products, mainly because they are potential sources of proteins, lipids, carbohydrates, and vitamins. In this way, the optimization of culture medium of these microorganisms can help to reduce production costs, making final products more accessible. In this sense, the present study aimed to investigate the effect of different trace metal concentrations on growth and biochemical composition of Scenedesmus obliquus. Cultures of S. obliquus were performed using the LCA-AD medium (pH 7.6), in 2-L cylindrical borosilicate glass flasks that were constantly stirred by bubbling 0.7% CO2 enriched air (v/v), irradiance ranging from 520 to 1200 μmols m−2 s−1, and temperature at 22 ± 1 °C. Four trace metal concentrations were tested: control (standard concentration used on Bold’s Basal Medium), ten (10−1), hundred (10−2), and thousand (10−3) times less than control. The trace metal concentration did not significantly affect the growth of S. obliquus – all treatments have reached about 4.2 g L−1 on the tenth day of cultivation. As well for growth performance, no significant differences were observed among the treatments for biomass composition percentages. The dry S. obliquus biomass was mainly composed of carbohydrates (about 65%), crude protein (about 20%), and finally lipids (about 12%). These findings suggest that up to 1000-fold reduction in trace metal concentration does not affect the growth and biochemical composition of the green microalga S. obliquus.



中文翻译:

微量金属对绿色微藻斜生藻生长性能及脂质,蛋白质和碳水化合物积累的影响

微藻生物质被认为是开发高商业价值产品的最有前途的原料之一,主要是因为它们是蛋白质,脂质,碳水化合物和维生素的潜在来源。这样,优化这些微生物的培养基可以帮助降低生产成本,使最终产品更容易获得。从这个意义上讲,本研究旨在研究不同微量金属浓度对凤尾鱼生长和生化成分的影响。的培养物S.斜使用LCA-AD培养基(pH7.6)中进行,在不断受到鼓泡0.7%CO 2下搅拌-L圆柱形硼硅酸盐玻璃烧瓶2富氧空气(V / V),辐照度范围从520到1200微摩尔-2  s -1,温度为22±1°C。测试了四种痕量金属浓度:对照(在Bold的基础培养基上使用的标准浓度),十倍(10 -1),百倍(10 -2)和千倍(10 -3)倍于对照。微量金属的浓度对斜纹链球菌的生长没有显着影响–在培养的第10天,所有处理均达到约4.2 g L -1。对于生长性能,在生物量组成百分比的处理之间未观察到显着差异。干燥斜S生物质主要由碳水化合物(约65%),粗蛋白(约20%)和最后的脂质(约12%)组成。这些发现表明,痕量金属浓度降低多达1000倍不会影响绿色微藻生链球菌的生长和生化组成。

更新日期:2020-03-23
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