The members of Streptomyces have been identified as a major source of antimicrobial agents with broad spectrum. This study is mainly focused on bioactivity-guided isolation and characterization of bioactive molecule from strain Streptomyces sp. T1317-0309 and its whole-genome sequence analysis for possible isolation of novel natural products. Strain Streptomyces sp. T1317-0309 showed 100% sequence similarity with strain Streptomyces lannensis TA4-8^T consisting 10, 453,255 bp of genome with 5 scaffolds and 69.9 mol% G + C content. The genome analyses revealed a total of 17 putative biosynthetic gene clusters (BGCs) responsible for various secondary metabolites including actinomycin, bacteriocin, ectoine, melanin, terpene, siderophore, betalactone, NRPS, T2PKS, and T3PKS. The BGC and bioactivity-guided purification of ethyl acetate extract of strain T1317-0309 showed the great potency of antimicrobial activities against various gram-positive multi-drug resistant human pathogens including MRSA. The BGC-predicted bioactive secondary metabolite was identified by various NMR analyses and confirmed as actinomycin D. In addition, this study reveals the first genome study of Streptomyces lannensis as a novel source for actinomycin D.

链霉菌的成员已被确定为广谱抗菌剂的主要来源。这项研究主要集中在菌株链霉菌（Streptomyces sp。）的生物活性指导的分离和生物活性分子的表征。T1317-0309及其全基因组序列分析，可用于分离新型天然产物。应变链霉菌属。T1317-0309与兰链霉菌TA4-8 ^T菌株显示100％的序列相似性包含10,453,255 bp的基因组，具有5个支架和69.9 mol％的G + C含量。基因组分析揭示了总共17个假定的生物合成基因簇（BGC），它们负责各种次级代谢产物，包括放线菌素，细菌素，菌素，黑色素，萜烯，铁载体，内酯，内酯，NRPS，T2PKS和T3PKS。BGC和T1317-0309菌株乙酸乙酯提取物的生物活性指导纯化显示出对各种革兰氏阳性多药耐药人类病原体（包括MRSA）的强大抗菌活性。BGC预测的生物活性次生代谢物通过各种NMR分析鉴定并确认为放线菌素D。此外，这项研究揭示了链球菌D作为放线菌素D的新来源的首次基因组研究。