ABSTRACT

Ideal methods for detecting pathogens should be sensitive, specific, rapid, cost-effective and instrument-free. Conventional nucleic acid pathogen detection strategies, mostly PCR-based techniques, have various limitations, such as expensive equipment, reagents and skilled performance. Recently, CRISPR/Cas-based methods have burst onto the scene, with the potential to power the pathogen detection field. Here we introduce these unique methods and discuss its hurdles and promises.

中文翻译：

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利用基于CRISPR / Cas的检测方法进行下一代病原体诊断。

摘要

检测病原体的理想方法应该灵敏，特异，快速，具有成本效益且无需仪器。常规的核酸病原体检测策略（主要是基于PCR的技术）具有各种局限性，例如昂贵的设备，试剂和熟练的性能。最近，基于CRISPR / Cas的方法应运而生，具有为病原体检测领域提供动力的潜力。在这里，我们介绍这些独特的方法并讨论其障碍和希望。