ABSTRACT

We investigated the differences in growth and rates of recurrence of the botryoid odontogenic cyst (BOC) and the less aggressive lateral periodontal cyst (LPC) and gingival cyst of the adult (GCA). We compared the immunohistochemical expression of selected biomarkers of apoptosis and proliferation and of regulators of their activity. Sections from archival paraffin blocks of 15 BOCs, six GCAs, six LPCs, and three odontogenic keratocysts (OKCs) were processed for immunohistochemical localization of Bcl-2, caspase-3, p53 and Ki-67. Labeled and unlabeled epithelial cells were counted and differences in the mean labeling index (LI) were evaluated statistically. The only significant differences in LI were for the anti-apoptotic marker, Bcl-2; the hierarchy was BOC > OKC > LPC > GCA. In two BOCs, 97% of the cells, and in all OKCs, all of the basal cells were labeled with Bcl-2. Otherwise, cells labeled with Bcl-2, p53 and caspase-3 were scattered among the basal and intermediate epithelial cell layers. Ki-67 labeled almost exclusively basal cells in the BOCs, LPCs and GCAs, and both basal and intermediate layer cells in the OKCs. Our findings suggest that while there was no significant difference in replicative potential of the GCAs, LPCs and BOCs, factors that influence apoptosis may be partially responsible for the more aggressive behavior of BOCs and OKCs.

摘要

我们研究了葡萄样牙源性囊肿 (BOC) 和侵袭性较低的外侧牙周囊肿 (LPC) 和成人牙龈囊肿 (GCA) 在生长和复发率方面的差异。我们比较了选定的细胞凋亡和增殖生物标志物及其活性调节剂的免疫组织化学表达。对来自 15 个 BOC、6 个 GCA、6 个 LPC 和三个牙源性角化囊 (OKC) 的存档石蜡块的切片进行处理，用于 Bcl-2、caspase-3、p53 和 Ki-67 的免疫组织化学定位。对标记和未标记的上皮细胞进行计数，并对平均标记指数 (LI) 的差异进行统计学评估。LI 的唯一显着差异是抗凋亡标记物 Bcl-2；层次结构是 BOC > OKC > LPC > GCA。在两个 BOC 中，97% 的单元格以及所有 OKC 中，所有的基底细胞都用 Bcl-2 标记。否则，用 Bcl-2、p53 和 caspase-3 标记的细胞分散在基底和中间上皮细胞层中。Ki-67 几乎只标记 BOC、LPC 和 GCA 中的基底细胞，以及 OKC 中的基底和中间层细胞。我们的研究结果表明，虽然 GCA、LPC 和 BOC 的复制潜力没有显着差异，但影响细胞凋亡的因素可能是 BOC 和 OKC 更具攻击性行为的部分原因。